S100 beta expression in Alzheimer's disease: relation to neuropathology in brain regions.
S100 beta expression in Alzheimer's disease: relation to neuropathology in brain regions.
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DOI：
10.1016/0167-4889(94)90101-5
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发表时间：
1994-09
期刊：
影响因子：
--
通讯作者：
L. V. Van Eldik;W. Griffin
中科院分区：
文献类型：
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作者：
L. V. Van Eldik;W. Griffin
采用S100β特异性ELISA和免疫组织化学分别检测了阿尔茨海默病（AD）患者表现出许多、一些、很少或没有神经斑块的脑区组织匀浆中的S100β水平和含有S100β的活化星形胶质细胞的分布。与在相似年龄的对照患者相似的死后时间间隔收集的样本相比，AD患者特定大脑区域的S100β水平升高，并且S100β的过表达与神经斑块的区域累及模式相对较好。S100β水平以海马和颞叶升高最多，额叶和脑桥次之，枕叶和小脑无升高。免疫组织化学分析显示S100β主要定位于神经斑块周围活化的星形胶质细胞。这些结果表明，S100β过表达是脑区域特异性的，与星形胶质细胞激活有关，并提示S100β高于一定阈值与阿尔茨海默病不同脑区域的神经病理受损伤程度有关。
S100β levels in tissue homogenates and distribution of S100β-containing activated astrocytes were examined by S100β-specific ELISA and immunohistochemistry, respectively, in brain regions exhibiting many, some, few, or no neuritic plaques in Alzheimer's disease (AD). Compared to samples collected at similar postmortem intervals from control patients of similar ages, S100β levels were elevated in specific brain regions from AD patients, and the overexpression of S100β correlated relatively well with the pattern of regional involvement by neuritic plaques. The largest increases in S100β levels were in hippocampus and temporal lobe, followed by frontal lobe and pons, with no elevation in occipital lobe or cerebellum. Immunohistochemical analysis showed S100β localization primarily in activated astrocytes surrounding neuritic plaques. These results demonstrate that S100β overexpression is brain region-specific and related to astrocyte activation and suggest that elevation of S100β above some threshold is related to the degree of neuropathological involvement of different brain regions in AD.