Teriparatide increases the maturation of circulating osteoblast precursors
Teriparatide increases the maturation of circulating osteoblast precursors
复制标题
DOI：
10.1007/s00198-011-1666-2
复制
发表时间：
2012-04-01
影响因子：
4
通讯作者：
Isaia, G. C.
中科院分区：
文献类型：
--
作者：
D'Amelio, P.;Tamone, C.;Isaia, G. C.
这项研究表明，特立哌酮促进受绝经后骨质疏松症影响的患者的循环成骨细胞（OB）前体的成熟程度。特立哌酮的合成代谢治疗已被证明对绝经后骨质疏松症的治疗有效，并显着降低非椎骨脆性骨折的风险。本研究的目的是探讨特立哌酮对循环OB前体的影响。我们通过流式细胞术和实时PCR评估了特立哌酮加钙和维生素D、雷洛昔芬加钙和维生素D或钙和维生素D单独治疗期间外周血单个核细胞中OB典型标志物在不同时间点的表达。血清骨碱性磷酸酶和骨钙素（OC）作为骨转换的标志物进行了测定，我们的研究结果表明，循环OB前体更多，更不成熟的脆性骨折患者比非骨折的骨质疏松患者。我们还表明，teriparbestrol治疗增加碱性磷酸酶和OC的OB前体的表达，因此，它增加了他们的成熟度。我们认为，teriparbestrol作为合成代谢剂也通过促进OB前体的成熟。
This study shows that teriparatide promotes the circulating osteoblast (OB) precursor degree of maturation in patients affected by postmenopausal osteoporosis.Anabolic treatment with teriparatide has proven effective for the therapy of postmenopausal osteoporosis and significantly reduces the risk of non-vertebral fragility fractures. The aim of this study was to investigate the effect of teriparatide on circulating OB precursors.We evaluated by flow cytometry and real-time PCR the expression of OBs typical markers in peripheral blood mononuclear cells during treatment with teriparatide plus calcium and vitamin D, raloxifene plus calcium and vitamin D or calcium and vitamin D alone at various time points. Serum bone alkaline phosphatase and osteocalcin (OC) were measured as markers of bone turnover.Our results show that circulating OB precursors are more numerous and more immature in patients affected by fragility fractures than in osteoporotic patients without fractures. We also show that teriparatide treatment increases the expression of alkaline phosphatase and of OC in OB precursors; thus, it increases their degree of maturation.We suggest that teriparatide acts as anabolic agents also by promoting the maturation of OB precursors.