Transcriptome Profile Analysis of Mechanisms of Black and White Plumage Determination in Black-Bone Chicken
Transcriptome Profile Analysis of Mechanisms of Black and White Plumage Determination in Black-Bone Chicken
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DOI：
10.1159/000489644
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发表时间：
2018-01-01
影响因子：
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通讯作者：
Sun, Wenxing
中科院分区：
文献类型：
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作者：
Yu, Shigang;Wang, Gang;Sun, Wenxing
背景/目标：黑色素是羽毛颜色的主要和普遍存在的成分，鸟类的黑色素沉着模式极其多样。然而，鸟类羽毛中色素沉着的分子机制仍然是未知的。研究方法：为了阐明黑色和白色羽毛形成的分子机制，本研究利用高通量测序技术比较了黑色和白色鸡毛球转录组的差异。本研究共构建了6个沐川乌骨鸡背羽中黑色（B组）和白色（W组）羽球的cDNA文库。结果如下：组B和W之间的比较揭示了61个差异表达的基因，其中47个在白色羽毛球中显示较高的表达水平，14个显示较低的表达水平。我们的研究结果揭示了一组候选基因和两个潜在的代谢途径参与乌骨鸡羽毛黑素合成。这些包括四个同源异型盒基因（HOXB 9，HOXC 8，HOXA 9和HOXC 9），两个谷胱甘肽（GSH）代谢相关基因（CHAC 1和GPX 3）和转化生长因子β（TGF-β）信号通路。两个已知的基因，TYR和MITF，也被证明在黑色素形成中起作用。结论：我们的数据为发现羽毛黑色素形成的重要基因提供了宝贵的资源，并将有助于进一步阐明黑色和白色羽毛的分子机制。(C)2018作者（S）由S发布。Karger AG，巴塞尔
Background/Aims: Melanin is a major and ubiquitous component of plumage colouration, and patterns of melanin pigmentation in birds are extremely varied. However, the molecular mechanism of pigmentation in avian plumage is still largely unknown. Methods: To elucidate the molecular mechanisms involved in the formation of black and white plumage, this study takes advantage of high-throughput sequencing technology to compare differences in the transcriptome between black and white chicken feather bulbs. In total, we constructed six cDNA libraries from black (Group B) and white (Group W) feather bulbs in the dorsal plumage of Muchuan black-boned chickens. Results: A comparison between Groups B and W revealed 61 differentially expressed genes, with 47 displaying higher, and 14 displaying lower, levels of expression in white feather bulbs. Our results revealed a set of candidate genes and two potential metabolic pathways involved in black-bone chicken plumage melanogenesis. These include four homeobox genes (HOXB9, HOXC8, HOXA9, and HOXC 9), two glutathione (GSH) metabolism-related genes (CHAC1 and GPX3), and the transforming growth factor beta (TGF-beta) signalling pathway. Two known genes, TYR and MITF, were also shown to play a role in melanin formation. Conclusion: our data provide a valuable resource for discovering genes important in plumage melanin formation and will help further elucidate the molecular mechanisms for black and white plumage. (C) 2018 The Author(s) Published by S. Karger AG, Basel