Characterisation, analysis of expression and localisation of circadian clock genes from the perspective of photoperiodism in the aphid Acyrthosiphon pisum
Characterisation, analysis of expression and localisation of circadian clock genes from the perspective of photoperiodism in the aphid Acyrthosiphon pisum
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DOI：
10.1016/j.ibmb.2017.02.006
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发表时间：
2017-04-01
影响因子：
3.8
通讯作者：
Martinez-Torres, David
中科院分区：
文献类型：
--
作者：
Barbera, Miguel;Mariano Collantes-Alegre, Jorge;Martinez-Torres, David
蚜虫是典型的光周期性昆虫，其从长日照季节典型的胎生孤雌生殖转变为卵生有性生殖，这是由秋季光周期缩短引发的，产生越冬卵，其中胚胎滞育发生。虽然生物钟基因参与哺乳动物的光周期性已经得到了很好的证实，但它们在昆虫中的参与仍然存在一些争议。豌豆蚜Acyrthosiphon pisum的基因组的可用性将该物种作为一个很好的模型来研究蚜虫季节反应中昼夜节律系统的参与。在本报告中，我们在生物钟基因的表征方面取得了进展，并表明这些基因显示出广泛的选择性剪接。此外，在一天的不同时刻分析的生物钟基因的表达，显示了中央时钟基因周期和永恒的强大循环。此外，这些基因的节律性表达被证明在DD（连续黑暗条件）下迅速减弱，从而支持基于严重减弱的昼夜节律振荡器的季节性反应模型。此外，在短日照条件下，一些生物钟基因的表达增加表明它们参与了蚜虫季节性反应的诱导。最后，在原位本地化的基因的转录物的周期和永恒的蚜虫大脑揭示了网站的时钟神经元的第一次在蚜虫。两组时钟细胞被确定：背神经元（DN）和侧神经元（LN），都在前脑。(C)2017爱思唯尔有限公司版权所有。
Aphids are typical photoperiodic insects that switch from viviparous parthenogenetic reproduction typical of long day seasons to oviparous sexual reproduction triggered by the shortening of photoperiod in autumn yielding an overwintering egg in which an embryonic diapause takes place. While the involvement of the circadian clock genes in photoperiodism in mammals is well established, there is still some controversy on their participation in insects. The availability of the genome of the pea aphid Acyrthosiphon pisum places this species as an excellent model to investigate the involvement of the circadian system in the aphid seasonal response. In the present report, we have advanced in the characterisation of the circadian clock genes and showed that these genes display extensive alternative splicing. Moreover, the expression of circadian clock genes, analysed at different moments of the day, showed a robust cycling of central clock genes period and timeless. Furthermore, the rhythmic expression of these genes was shown to be rapidly dampened under DD (continuous darkness conditions), thus supporting the model of a seasonal response based on a heavily dampened circadian oscillator. Additionally, increased expression of some of the circadian clock genes under short-day conditions suggest their involvement in the induction of the aphid seasonal response. Finally, in situ localisation of transcripts of genes period and timeless in the aphid brain revealed the site of clock neurons for the first time in aphids. Two groups of clock cells were identified: the Dorsal Neurons (DN) and the Lateral Neurons (LN), both in the protocerebrum. (C) 2017 Elsevier Ltd. All rights reserved.