An ultrasensitive microfluidic chip-based immunoassay for multiplex determination of 11 PDE-5 inhibitors in adulterated health foods
An ultrasensitive microfluidic chip-based immunoassay for multiplex determination of 11 PDE-5 inhibitors in adulterated health foods
复制标题
基于超灵敏微流控芯片的免疫分析法多重测定掺假保健食品中的 11 种 PDE-5 抑制剂
DOI：
10.1016/j.snb.2022.131450
复制
发表时间：
2022-02-02
影响因子：
8.4
通讯作者：
Lei, Hongtao
中科院分区：
文献类型：
--
作者：
Guan, Tian;Shen, Yudong;Lei, Hongtao
随着保健食品中非法添加物（西地那非和他达拉非掺假物）等多种污染物的同时出现，食品的多类化学污染问题日益受到关注。在本研究中，一个超灵敏的多重微流控芯片检测西地那非和他达拉非掺假。基于我们制备的明亮荧光信号报告子、有效的基于微流控芯片的分析物富集策略和移动的检测平台，获得了显著增强的灵敏度和测定便利性。在最佳条件下，该方法可检出11种西地那非和他达拉非混伪品，检出限为0.027-0.066 ng/mL，检出限为2.0-9.0 ng/mL。由于合理的回收率（他达拉非为94.3%-99.8%，西地那非为87.8%-112%）和可接受的变异系数（2.81%-11.2%），验证了高准确度和精密度。本工作和LC-MS/MS分析之间的比较实验发现，在胶囊，茶叶，速溶咖啡和保健酒样品中的总西地那非和他达拉非掺假物的同时筛查良好的协议。这种新的检测形式可以广泛用于在几分钟内对多种PDE-5抑制剂进行超灵敏现场检测，同时还提供了一种易于操作和低成本的多重检测方案。
Multiclass chemical contamination of food has aroused growing attention due to the increasingly co-occurrence of multiple contaminations, such as illegal additives (sildenafil and tadalafil adulterants) in health food. In the present study, an ultrasensitive multiplex microfluidic chip-based assay towards sildenafil and tadalafil adulterants was developed. Dramatically enhanced sensitivity and assay convenience were obtained based on our prepared brightly fluorescent signal reporters, effective microfluidic chip-based analyte enrichment strategy and a mobile detection platform. Under optimal conditions, this method could detect 11 sildenafil and tadalafil adulterants with low limits of detection (0.027-0.066 ng/mL) and low cut-off values (2.0-9.0 ng/mL). High accuracy and precision were validated due to the reasonable recoveries (94.3%-99.8% for tadalafil, 87.8-112% for sildenafil) and acceptable coefficient of variation (2.81-11.2%). Comparison experiments between this work and LC-MS/MS analysis found good agreement in the simultaneous screening of total sildenafil and tadalafil adulterants in capsule, tea leaves, instant coffee and health wine samples. This new assay format can be extensively employed in ultrasensitive field detection of multiple PDE-5 inhibitors within minutes, while also providing an easy-to-operate and low-cost multiplex detection protocol.