Resonance Energy Transfer between Dye Molecules in Colloids of a Layered Silicate. The Effect of Dye Surface Concentration
Resonance Energy Transfer between Dye Molecules in Colloids of a Layered Silicate. The Effect of Dye Surface Concentration
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DOI：
10.1021/acs.jpcc.7b00947
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发表时间：
2017-04-20
影响因子：
3.7
通讯作者：
Bujdak, J.
中科院分区：
文献类型：
--
作者：
Belusakova, S.;Martinez-Martinez, V.;Bujdak, J.
由皂石型的合成层状硅酸盐（Sap）和两种阳离子激光染料罗丹明6 G（R6 G）和恶嗪4（Ox 4）制备胶体分散体。染料分子在Sap颗粒上的吸附既不导致分子聚集，也不导致分子分离。福斯特共振能量转移（FRET），研究使用稳态和时间分辨荧光（TRF）光谱，发生从R6 G到Ox 4，和效率的增加与吸附的染料分子的表面浓度，确定的染料/Sap比。在定义分子间距离概率密度函数的基础上建立了FRET的理论模型。理论模型与实验结果吻合得很好。分子的扩散可能有助于FRET效率的增加，特别是在较低的染料浓度，而各向异性因素的影响是最有可能忽略不计。
Colloidal dispersions were prepared from a synthetic layered silicate of saponite type (Sap) and two cationic laser dyes, rhodamine 6G (R6G) and oxazine 4 (Ox4). The adsorption of dye molecules on Sap particles led to neither molecular aggregation nor segregation of dye molecules. Forster resonance energy transfer (FRET), investigated using steady-state and time-resolved fluorescence (TRF) spectroscopies, occurs from R6G to Ox4, and the efficiency increased with the surface concentration of adsorbed dye molecules, as determined by the dye/Sap ratio. Theoretical modeling of FRET based on defining probability density functions of intermolecular distances is presented. The theoretical model and experimental results were in very good agreement. Diffusion of the molecules might have contributed to the increase of FRET efficiency, especially at lower dye concentrations, whereas the influence of anisotropy factors was most likely negligible.