Interactions of GM1 Ganglioside with Crude Rat Brain Neuronal Membranes
Interactions of GM1 Ganglioside with Crude Rat Brain Neuronal Membranes
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GM1 神经节苷脂与粗制大鼠脑神经元膜的相互作用
DOI：
10.1111/j.1471-4159.1980.tb07083.x
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发表时间：
1980
影响因子：
4.7
通讯作者：
G. Tettamanti
中科院分区：
文献类型：
--
作者：
G. Toffano;D. Benvegnù;A. Bonetti;L. Facci;A. Leon;P. Orlando;R. Ghidoni;G. Tettamanti
研究了 GM1、神经节苷脂与大鼠脑神经元膜粗制剂的结合，获得了以下结果：（a）结合过程遵循双相动力学，在 0.07–0.08 X 10−6m GM1 浓度时显示断裂； (b) GM1 处的结合过程特征、断裂下方的浓度和断裂上方 10-6m 上方的浓度似乎有所不同。在断裂以下，该过程缓慢进行，并使 GM1 分子与膜形成稳定且不可逆的结合。超过 10-6m，该过程要快得多，并导致 GM1 分子相互作用，使得它们可以通过洗涤释放，并可以与新添加的游离神经节苷脂交换； (c) 两个结合过程表现出饱和现象的特征； (d) 在这两种情况下，所摄取的 GM1 可自由地被半乳糖氧化酶利用，表明寡糖链从膜表面突出。我们假设 GM1 以不同的物理形式出现在断裂的下方和上方，每种形式都具有不同的与膜相互作用的机制。在 10-6m GM1 以上，以胶束形式相互作用，胶束-膜相互作用的基础是融合过程。在断裂点以下，在 10−8–10−7m 范围内，结合是膜上位点与单个神经节苷脂分子（最有可能以单体形式）的疏水部分之间疏水相互作用的结果。托法诺 G. 等人。 GM1、神经节苷脂与粗制大鼠脑神经元膜的相互作用。 J. Neurochem.35, 861–866 (1980)。
The binding of GM1, ganglioside to crude preparations of rat brain neuronal membranes was studied, the following results being obtained: (a) the binding process followed a biphasic kinetics, which displayed a break at 0.07–0.08 X 10−6m GM1, concentration; (b) the features of the binding process at GM1, concentrations below the break and, over the break, above 10‐6m appeared to be different. Below the break the process proceeded slowly and brought a stable and irreversible association of GM1, molecules to the membranes. Over 10‐6m the process was much more rapid and caused GM1, molecules to interact in such a way that they were releasable by washing and could exchange with newly added free ganglioside; (c) the two binding processes displayed the characteristics of a saturation phenomenon; (d) in both cases, GM1, taken up was freely available to galactose oxidase, indicating that the oligosaccharide chains protrude from the membrane surface. We postulate that GM1, occurs, below and above the break, in different physical forms, each of them having a different mechanism of interaction with the membrane. Above 10‐6m GM1, interacts as micelles, and the basis of the micelle‐membrane inter action is a fusion process. Below the break, in the 10−8–10−7m range, the binding is the result of hydrophobic interactions between sites on the membrane and the hydrophobic portion of individual ganglioside molecules, most likely in the monomeric form. Toffano G. et al. Interactions of GM1, ganglioside with crude rat brain neuronal membranes. J. Neurochem.35, 861–866 (1980).