Induction of an increase in mitochondrial matrix enzymes in muscle of iron-deficient rats.
Induction of an increase in mitochondrial matrix enzymes in muscle of iron-deficient rats.
复制标题
诱导缺铁大鼠肌肉中线粒体基质酶的增加。
DOI：
10.1152/ajpcell.1987.253.5.c639
复制
发表时间：
1987
期刊：
影响因子：
--
通讯作者：
Holloszy,JO
中科院分区：
文献类型：
--
作者：
Ohira,Y;Cartier,LJ;Chen,M;Holloszy,JO
维持缺铁饮食的幼鼠会出现严重的贫血，骨骼肌线粒体呼吸链的含铁黄素蛋白和细胞色素水平大幅下降。相反，在红色骨骼肌中，柠檬酸合成酶、异柠檬酸脱氢酶、3-羟基酰辅酶A脱氢酶、3-酮酸-辅酶A转移酶和天冬氨酸氨基转移酶等线粒体基质标志物酶的水平在红色骨骼肌中增加，而在白色肌肉中没有。冷冻后比目鱼肌中磷酸肌酸浓度降低，无机磷浓度升高。我们假设，线粒体基质酶的增加反映了对严重缺铁大鼠维持姿势和负重红色肌肉纤维中线粒体生物发生的刺激。我们的工作假设是，这种对线粒体生物发生的刺激来自红色纤维的轻微活动，并且是由于通常由剧烈运动或低氧引起的细胞内稳态的扰动。在缺铁的情况下，对线粒体生物发生的刺激只能诱导那些没有被缺铁阻止的酶的增加，导致组成严重异常的线粒体的形成。
Young rats maintained on an iron-deficient diet developed severe anemia and had large decreases in the levels of the iron-containing flavoproteins and cytochromes of the mitochondrial respiratory chain in skeletal muscle. In contrast, the levels of a number of mitochondrial matrix marker enzymes, including citrate synthase, isocitrate dehydrogenase, 3-hydroxyacyl-CoA dehydrogenase, 3-ketoacid-CoA transferase, and aspartate aminotransferase, increased in red skeletal muscle but not in white muscle. Phosphocreatine concentration was decreased and inorganic phosphate concentration was increased in soleus muscle frozen in situ. We hypothesize that the increase in mitochondrial matrix enzymes reflects a stimulus to mitochondrial biogenesis in posture-maintaining and weight-bearing red muscle fibers in severely iron-deficient rats. It is our working hypothesis that this stimulus to mitochondrial biogenesis arises from mild activity of the red fibers and is due to the same perturbation in cellular homeostasis that is normally caused by vigorous exercise or hypoxia. In iron deficiency, the stimulus to mitochondrial biogenesis can induce an increase in only those enzymes not prevented from increasing by iron deficiency, resulting in formation of mitochondria of grossly abnormal composition.