Characterization of hepatitis B virus core mutants that inhibit viral replication.
Characterization of hepatitis B virus core mutants that inhibit viral replication.
复制标题
抑制病毒复制的乙型肝炎病毒核心突变体的表征。
DOI：
10.1006/viro.1994.1625
复制
发表时间：
1994
期刊：
影响因子：
3.7
通讯作者：
Wands,JR
中科院分区：
文献类型：
--
作者：
Scaglioni,PP;Melegari,M;Wands,JR
我们已经产生了嗜肝DNA病毒的显性阴性核心蛋白变体，并对其功能进行了表征，以确定其对“野生型”病毒复制的影响。通过瞬时转染将表达这些构建体的质粒引入肝癌细胞系中，并通过纯化的病毒核心颗粒的Southern印迹分析来评估对野生型土拨鼠肝炎病毒（WHV）和B肝炎病毒（HBV）复制的影响。WHV和HBV构建体表达与小表面蛋白的C-末端框内融合的截短核心蛋白，发现由于病毒核衣壳组装过程的破坏和防止前基因组RNA的降解而抑制90-95%的重要复制。发现抗病毒作用对靶向病毒具有特异性。这些结果表明嗜肝DNA病毒核心蛋白的突变体可能代表一类新的抗病毒剂。K的;
We have generated and functionally characterized dominant negative core protein variants of the hepadnaviruses to determine their effects on "wild type" viral replication. Plasmids expressing these constructs were introduced into hepatoma cell lines by transient transfection and effects on wild type woodchuck hepatitis virus (WHV) and hepatitis B virus (HBV) replication were evaluated by Southern blot analysis of purified viral core particles. WHV and HBV constructs expressing a truncated core protein fused in frame with the C-terminus of the small surface protein were found to inhibit vital replication by 90-95% due to disruption of the viral nucleocapsid assembly process and preventing encapsidation of pregenomic RNA. The antiviral effects were found to be specific for the targeted virus. These results demonstrate that mutants of hepadnaviral core protein may represent a novel class of antiviral agents. K;