Transcriptional profiling of Francisella tularensis infected peripheral blood mononuclear cells: a predictive tool for tularemia.
Transcriptional profiling of Francisella tularensis infected peripheral blood mononuclear cells: a predictive tool for tularemia.
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土拉弗朗西斯菌感染的外周血单核细胞的转录谱：土拉菌病的预测工具。
DOI：
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发表时间：
2008
影响因子：
--
通讯作者：
L. DaSilva
中科院分区：
文献类型：
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作者：
C. Paranavitana;P. Pittman;Mahendran Velauthapillai;E. Zelazowska;L. DaSilva
在这项研究中，我们分析了时间的基因表达模式，在人外周血单核细胞（PBMC）感染弗朗西斯菌活疫苗株从1至24小时，利用全人类Affygene芯片。我们发现，相当数量的诱导基因具有相似的表达模式和功能，如先前报道的溃疡腺土拉菌病患者的基因表达谱。在报告的兔热病患者作为警报信号基因簇的一部分的六个独特调控的基因中，五个，即半胱天冬酶1，PSME 2，TAP-1，GBP 1和GCH 1，在体外诱导。我们还检测了兔热病患者报告的7种潜在生物标志物中的4种，即4小时的TNFAIP 6和16和24小时的STAT 1，TNFSF 10和SECTM 1。这些观察结果强调了使用微阵列表达谱作为体外工具来鉴定人类感染和疾病的潜在生物标志物的价值。我们的研究结果表明，弗朗西斯菌感染的几个宿主途径/过程的潜在参与，特别是那些参与钙，锌离子结合，过氧化物酶体激活受体信号传导和脂质代谢，这进一步完善了目前的知识，F。土拉菌感染及其对人类宿主的影响。最终，这项研究提供了支持，利用在体外微阵列基因表达谱在人类PBMC中，以确定感染的生物标志物和预测在体内的免疫反应的感染剂。
In this study, we analyzed temporal gene expression patterns in human peripheral blood mononuclear cells (PBMCs) infected with the Francisella tularensis live vaccine strain from 1 to 24 h utilizing a whole human Affymetrix gene chip. We found that a considerable number of induced genes had similar expression patterns and functions as reported previously for gene expression profiling in patients with ulceroglandular tularemia. Among the six uniquely regulated genes reported for tularemia patients as being part of the alarm signal gene cluster, five, namely caspase 1, PSME2, TAP-1, GBP1, and GCH1, were induced in vitro. We also detected four out of the seven potential biomarkers reported in tularemia patients, namely TNFAIP6 at 4 h and STAT1, TNFSF10, and SECTM1 at 16 and 24 h. These observations underscore the value of using microarray expression profiling as an in vitro tool to identify potential biomarkers for human infection and disease. Our results indicate the potential involvement of several host pathways/processes in Francisella infection, notably those involved in calcium, zinc ion binding, PPAR signaling, and lipid metabolism, which further refines the current knowledge of F. tularensis infection and its effects on the human host. Ultimately, this study provides support for utilizing in vitro microarray gene expression profiling in human PBMCs to identify biomarkers of infection and predict in vivo immune responses to infectious agents.
DOI：
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发表时间：
1998
影响因子：
11.1
作者：
S. Der;A. Zhou;B. Williams;R. Silverman
通讯作者：
S. Der;A. Zhou;B. Williams;R. Silverman
影响因子：
15.9
作者：
Gewirtz, AT;Rao, AS;Neish, AS
通讯作者：
Neish, AS
影响因子：
20.3
作者：
Zhou, QS;Zhao, J;Sims, PJ
通讯作者：
Sims, PJ