Changes in the pattern of aldehyde dehydrogenase activity in primary and metastatic adenocarcinomas of the human colon.
Changes in the pattern of aldehyde dehydrogenase activity in primary and metastatic adenocarcinomas of the human colon.
复制标题
人类结肠原发性和转移性腺癌中乙醛脱氢酶活性模式的变化。
DOI：
10.1016/0304-3835(87)90070-x
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发表时间：
1987
期刊：
影响因子：
9.7
通讯作者：
Michalopoulos,G
中科院分区：
文献类型：
--
作者：
Marselos,M;Michalopoulos,G
本文测定了原发性和转移性结肠腺癌组织中乙醛脱氢酶（ALDH）的活性。用丙醛和NAD（P-NAD）或苯甲醛和NADP（B-NADP）测定酶。原发性结肠腺癌的P-NAD活性水平与癌旁正常粘膜相似，但B-NADP活性相对较高。转移性结肠腺癌肝组织P-NAD活性低于正常肝组织，B-NADP活性高于正常肝组织。正常结肠粘膜中ALDH的水平一般低于肝脏。低温组织切片的组织化学染色显示，该酶仅限于结肠粘膜腺管的上皮细胞。这可以解释，沿着显著的粘膜间变性改变，在原发性和转移性腺癌的匀浆中检测到ALDH活性的变化。然而，在腺癌与邻近的正常组织中的B-NADP活性的比率是恒定的和显着的高。
The activity of aldehyde dehydrogenase (ALDH) was measured in primary and metastatic human colonic adenocarcinomas. The enzyme was determined with either propionaldehyde and NAD (P-NAD) or with benzaldehyde and NADP (B-NADP). Primary colonic adenocarcinomas had levels of P-NAD activity similar to those of the adjacent normal mucosa, but relatively higher B-NADP activity. Also the metastatic colonic adenocarcinomas of the liver had lower P-NAD and higher B-NADP activity than the normal hepatic parenchyma. The level of ALDH was in general lower in the normal colonic mucosa, when compared to the liver. Histochemical staining of cryostat tissue sections revealed that the enzyme is exclusively confined to the epithelial cells of the glandular ducts of the colonic mucosa. This may explain, along with the significant anaplastic changes of the mucosa, the variation of ALDH activity detected in the homogenates of the primary and metastatic adenocarcinomas. However, the ratio of B-NADP activity in the adenocarcinomas versus the adjacent normal tissues was constantly and significantly high.