Effect of pulse duration on two-photon excited fluorescence and second harmonic generation in nonlinear optical microscopy
Effect of pulse duration on two-photon excited fluorescence and second harmonic generation in nonlinear optical microscopy
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DOI：
10.1117/1.2177676
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发表时间：
2006-03-01
影响因子：
3.5
通讯作者：
Tromberg, Bruce J.
中科院分区：
文献类型：
--
作者：
Tang, Shuo;Krasieva, Tatiana B.;Tromberg, Bruce J.
我们已经开发了一个多光子显微镜（MPM）系统，使用12-fs的钛宝石激光器与可调色散预补偿，以研究脉冲宽度对非线性光学信号的影响。双光子激发荧光（TPEF）和二次谐波产生（SHG）的效率进行了研究，在样品处测量的各种脉冲持续时间，范围从类似的400 fs到子20 fs。在整个测试范围内，TPEF和SHG均与脉冲持续时间的倒数成比例增加。由于改善的信噪比，与120-fs脉冲相比，亚20-fs脉冲用于在人体皮肤中将MPM成像深度增强约160%。(c)2006年，由光学仪器工程师协会（Society of Photo-Optical Instrumentation Engineers）主办。
We have developed a multiphoton microscopy (MPM) system using a 12-fs Ti:sapphire laser with adjustable dispersion precompensation in order to examine the impact of pulse duration on nonlinear optical signals. The efficiencies of two-photon-excited fluorescence (TPEF) and second harmonic generation (SHG) were studied for various pulse durations, measured at the sample, ranging from similar to 400 fs to sub-20 fs. Both TPEF and SHG increased proportionally to the inverse of the pulse duration for the entire tested range. Because of improved signal-to-noise ratio, sub-20-fs pulses were used to enhance MPM imaging depth by approximately 160%, compared to 120-fs pulses, in human skin. (c) 2006 Society of Photo-Optical Instrumentation Engineers.