NAFLD and Atherosclerosis Are Prevented by a Natural Dietary Supplement Containing Curcumin, Silymarin, Guggul, Chlorogenic Acid and Inulin in Mice Fed a High-Fat Diet.
NAFLD and Atherosclerosis Are Prevented by a Natural Dietary Supplement Containing Curcumin, Silymarin, Guggul, Chlorogenic Acid and Inulin in Mice Fed a High-Fat Diet.
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DOI：
10.3390/nu9050492
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发表时间：
2017-05-13
期刊：
影响因子：
5.9
通讯作者：
Di Carlo M
中科院分区：
文献类型：
--
作者：
Amato A;Caldara GF;Nuzzo D;Baldassano S;Picone P;Rizzo M;Mulè F;Di Carlo M
非酒精性脂肪肝（NAFLD）会增加心血管疾病的风险。NAFDL与致动脉粥样硬化性血脂异常、炎症和肾素-血管紧张素系统（RAS）失衡相关，进而导致动脉粥样硬化病变。在本研究中，评价了含有姜黄、水飞蓟素、guggul、绿原酸和菊粉的天然膳食补充剂（NDS）对NAFLD和动脉粥样硬化的影响，并研究了其作用机制。C57 BL/6小鼠喂食HFD 16周;一半小鼠同时每日经口给予（os）NDS。评价了NAFLD和主动脉和颈动脉中的致动脉粥样硬化病变（组织学分析）、涉及NAFLD的基因的肝脏表达（PCR阵列）、肝脏血管紧张素原（AGT）和AT 1 R mRNA表达（实时PCR）以及血浆血管紧张素（ANG）-II水平（ELISA）。在NDS组中，未观察到脂肪变性、主动脉病变或颈动脉增厚。PCR阵列显示参与脂质代谢和抗炎活性的一些基因（Cpt 2，Ifng）上调，参与促炎反应和游离脂肪酸摄取的一些基因（Fabp 5，Socs 3）下调。肝脏AGT、AT 1 R mRNA和ANG II血浆水平均显著低于未治疗组。此外，NDS抑制未处理动物中观察到的血脂异常。总之，这些结果表明，NDS通过调节参与NAFLD的不同基因的表达和避免RAS失衡来预防NAFLD和动脉粥样硬化形成。
Non-alcoholic fatty liver disease (NAFLD) confers an increased risk of cardiovascular diseases. NAFDL is associated with atherogenic dyslipidemia, inflammation and renin-angiotensin system (RAS) imbalance, which in turn lead to atherosclerotic lesions. In the present study, the impact of a natural dietary supplement (NDS) containing Curcuma longa, silymarin, guggul, chlorogenic acid and inulin on NAFLD and atherosclerosis was evaluated, and the mechanism of action was examined. C57BL/6 mice were fed an HFD for 16 weeks; half of the mice were simultaneously treated with a daily oral administration (os) of the NDS. NAFLD and atherogenic lesions in aorta and carotid artery (histological analysis), hepatic expression of genes involved in the NAFLD (PCR array), hepatic angiotensinogen (AGT) and AT1R mRNA expression (real-time PCR) and plasma angiotensin (ANG)-II levels (ELISA) were evaluated. In the NDS group, steatosis, aortic lesions or carotid artery thickening was not observed. PCR array showed upregulation of some genes involved in lipid metabolism and anti-inflammatory activity (Cpt2, Ifng) and downregulation of some genes involved in pro-inflammatory response and in free fatty acid up-take (Fabp5, Socs3). Hepatic AGT, AT1R mRNA and ANG II plasma levels were significantly lower with respect to the untreated-group. Furthermore, NDS inhibited the dyslipidemia observed in the untreated animals. Altogether, these results suggest that NDS prevents NAFLD and atherogenesis by modulating the expression of different genes involved in NAFLD and avoiding RAS imbalance.