CHROMOSOMAL ABERRATION AND SISTER-CHROMATID EXCHANGE FREQUENCIES IN PERIPHERAL-BLOOD LYMPHOCYTES OF A LARGE HUMAN-POPULATION SAMPLE
CHROMOSOMAL ABERRATION AND SISTER-CHROMATID EXCHANGE FREQUENCIES IN PERIPHERAL-BLOOD LYMPHOCYTES OF A LARGE HUMAN-POPULATION SAMPLE
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DOI：
10.1016/0165-1218(88)90038-9
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发表时间：
1988-03-01
期刊：
影响因子：
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通讯作者：
SHELBY, MD
中科院分区：
文献类型：
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作者：
BENDER, MA;PRESTON, RJ;SHELBY, MD
为了评估外周血淋巴细胞的细胞遗传学测定作为监测受低水平职业和环境暴露于化学诱变剂和致癌物的人群的平均值的潜力，需要准确的基线数据。因此，我们在布鲁克海文国家实验室的353名健康员工中确定了染色体畸变和姐妹染色单体交换的平均频率，它们的方差，以及这种方差的来源。血样、淋巴细胞培养、细胞遗传学准备和评分采用了详细的方案，以尽量减少这些潜在来源的变异。评分分为橡树岭组和布鲁克海文组，重复评分足以评估和最小化个体评分者之间任何差异的影响。总的来说，这些数据包括71,950个染色体畸变细胞和16,898个姐妹染色单体交换细胞。姐妹染色单体交换的平均调整频率为8.29 +-。0.08细胞。据其他研究报告，吸烟对姐妹染色单体交换频率影响很大；在我们的研究中，吸烟者的平均值为9.0 +-。0.2，而非吸烟者为8.1 +-。0.1 /细胞。与吸烟状况无关，女性的平均发病频率在统计学上高于男性。另一方面，受试者的年龄对姊妹染色单体交换频率没有显著影响。奇怪的是，受试者的白血球总数确实影响姊妹染色单体交换频率。没有发现其他变异来源。测定所有类型染色体畸变的频率。最常见的明确染色单体类型，即染色单体缺失的频率为0.81 +-。0.05%，最常见的明确染色体类型，双心染色体，为0.16 +-。0.02%。没有发现年龄或性别以及所测试的任何其他参数对任何染色体畸变类型的频率有统计学上显著的影响，只有一个例外是长无中心片段，通常是“多余的”，据信代表X染色体在着丝粒处过早分离。这类片段在女性样本中的出现频率明显高于男性样本，且与年龄呈显著正相关。
In order to assess the potential of cytogenetic determination on peripheral blood lymphocytes as a mean of monitoring human population subject to low level occupational and environmental exposures to chemical mutagens and carcinogens, accurate baseline data are required. Accordingly, we have determined mean frequencies of chromosomal aberrations and of sister-chromatid exchanges, their variances, and the sources of this variance in a cohort of 353 healthy employees of the Brookhaven National Laboratory. A detailed protocol was adopted for blood sampling, lymphocyte culture, cytogenetic preparation and scoring in order to minimize variation from these potential sources. Scoring was divided between the Oak Ridge and the Brookhaven groups with duplicate scoring sufficient to evaluate and minimize the effect of any differences between individual scorers. In all, the data include 71,950 cells scored for chromosomal aberrations and 16,898 cells scored for sister-chromatid exchanges. The mean adjusted frequency of sister-chromatid exchanges was 8.29 .+-. 0.08 cell. As reported in other studies, cigarette smoking very significantly influenced sister-chromatid exchange frequencies; in our study the mean for smokers was 9.0 .+-. 0.2, while that for non-smokers was 8.1 .+-. 0.1/cell. The mean frequency was statistically higher in females than in males, regardless of smoking status. On the other hand, age of the subject did not significantly influence sister-chromatid exchange frequencies. Curiously, the subject''s total white cell count did influence sister-chromatid exchange frequency. No other source of variation was found. The frequencies of chromosomal aberrations of all types were determined. The frequency of the most common unequivocal chromatid type, the chromatid deletion, was 0.81 .+-. 0.05%, that of the most common unequivocal chromosome type, the dicentric, was 0.16 .+-. 0.02%. No statistically significant influence was found of age or sex, nor of any other parameter tested, on the frequency of any chromosomal aberration type, with the single exception of long acentric fragments, often "supernumerary'', believed to represent X chromosomes precociously separated at the centromere. Such fragments were significantly more frequent in samples from females than those from males, and showed a significant positive regression on age.