Limnohabitans australis sp. nov., isolated from a freshwater pond, and emended description of the genus Limnohabitans.
Limnohabitans australis sp. nov., isolated from a freshwater pond, and emended description of the genus Limnohabitans.
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DOI：
10.1099/ijs.0.022384-0
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发表时间：
2010-12
影响因子：
2.8
通讯作者：
Šimek K
中科院分区：
文献类型：
--
作者：
Hahn MW;Kasalický V;Jezbera J;Brandt U;Šimek K
从巴西淡水池塘中分离到一株化能有机营养型、需氧、非运动型菌株MWH-BRAZ-DAM 2DT，对其进行了表型、遗传学和化学分类学研究。16 S rRNA基因序列的系统发育分析表明，该菌株隶属于属Limnohabitans（丛枝藻科，Betaproteobacteria）。该分离物与代表该属模式种的弯曲沼栖藻MWH-C5 T、小沼栖藻和南极沼栖藻模式株的16 SrRNA基因序列相似性分别为98.2%、96.5%和97.0%。 DNA-DNA再结合分析与DNA的模式株的所有三个先前描述的Limnohabitans物种显示的相似性值在26.2- 44.6%的范围内。 分离物的主要脂肪酸为C16：1ω 7 c/ω6c、C16：0、C12：0和C8：0 3-OH，主要醌为泛醌Q-8，DNA G+C含量为55.8mol%。 分离物可以区分从模式菌株的三个Limnohabitans物种的几个表型性状，包括在利用几种碳源的差异。根据该分离物的亲缘关系及其与三个最接近的物种的差异，该分离物代表了一个新种，并建议将其命名为Limnohabitans australis sp. nov.。模式菌株是MWH-BRAZ-DAM 2DT（=DSM 21646 T =CCUG 56719 T）。
A chemo-organotrophic, aerobic, non-motile strain, MWH-BRAZ-DAM2DT, isolated from a freshwater pond in Brazil, was characterized phenotypically, phylogenetically and chemotaxonomically. Phylogenetic analysis of 16S rRNA gene sequences indicated affiliation of the strain with the genus Limnohabitans (Comamonadaceae, Betaproteobacteria). 16S rRNA gene sequence similarities between the isolate and Limnohabitans curvus MWH-C5T, representing the type species of the genus, and the type strains of Limnohabitans parvus and Limnohabitans planktonicus were 98.2, 96.5 and 97.0 %, respectively. DNA–DNA reassociation analyses with DNA of the type strains of all three previously described Limnohabitans species revealed similarity values in the range 26.2–44.6 %. The predominant fatty acids of the isolate were C16 : 1ω7c/ω6c, C16 : 0, C12 : 0 and C8 : 0 3-OH, the major quinone was ubiquinone Q-8 and the DNA G+C content was 55.8 mol%. The isolate could be discriminated from the type strains of the three Limnohabitans species by several phenotypic traits including differences in the utilization of several carbon sources. Based on the phylogeny of the isolate and its differences from the three most closely related species, the isolate represents a novel species for which the name Limnohabitans australis sp. nov. is proposed. The type strain is MWH-BRAZ-DAM2DT (=DSM 21646T=CCUG 56719T).
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影响因子：
4.4
作者：
Hahn, MW;Pöckl, M
通讯作者：
Pöckl, M
影响因子：
4.4
作者：
Simek, K;Pernthaler, J;Amann, R
通讯作者：
Amann, R
影响因子：
5.1
作者：
Simek, Karel;Hornak, Karel;Hahn, Martin W.
通讯作者：
Hahn, Martin W.
影响因子：
4.4
作者：
Hahn, MW;Pöckl, M;Wu, QLL
通讯作者：
Wu, QLL
DOI：
10.1099/ijs.0.013292-0
发表时间：
2010-06
影响因子：
2.8
作者：
Hahn MW;Kasalický V;Jezbera J;Brandt U;Jezberová J;Šimek K
通讯作者：
Šimek K