Relation between rotation of MreB actin and cell width of Escherichia coli
Relation between rotation of MreB actin and cell width of Escherichia coli
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DOI：
10.1111/gtc.12667
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发表时间：
2019-03-01
期刊：
影响因子：
2.1
通讯作者：
Shiomi, Daisuke
中科院分区：
文献类型：
--
作者：
Kurita, Keisuke;Shin, Ryota;Shiomi, Daisuke
细菌细胞，包括大肠杆菌和枯草芽孢杆菌，不断延长和分裂。虽然细胞宽度在细胞周期中得到维持，但其调控的分子机制尚不清楚。MreB被认为在维持细胞宽度方面发挥作用。已经确定了mreB中影响细胞宽度的几个点突变。MreB蛋白在细胞内形成簇状或聚合物，并沿着垂直于长轴的环形轨道移动。这种旋转与肽聚糖合成相结合。在这里，我们专注于两个MreB突变体，MreB（A125V）和MreB（A174T）。产生MreB（A125V）和MreB（A174T）的细胞比WT细胞更薄和更厚，MreB（A125V）和MreB（A174T）的旋转速度分别比WT MreB快和慢。我们观察到旋转速率与细胞壁合成速率相关。因此，我们得出MreB旋转的速度也会影响单元格的宽度，即MreB旋转的速度越快，单元格的宽度越薄。
Bacterial cells, including Escherichia coli and Bacillus subtilis, continuously elongate and divide. Although the cell width is maintained during cell cycle, the molecular mechanisms involved in its regulation remain unknown. MreB has been implicated to play a role in maintaining cell width. Several point mutations in mreB that affect cell width have been identified. The MreB protein forms clusters or polymers in the cell and moves along annular tracks perpendicular to the long axis. This rotation is coupled with peptidoglycan synthesis. Here, we focused on two MreB mutants, MreB(A125V) and MreB(A174T). Cells producing MreB(A125V) and mreB(A174T) were thinner and thicker than WT cells, and MreB(A125V) and MreB(A174T) rotated faster and slower than WT MreB, respectively. We observed that the rotation rate correlated with the cell wall synthesis rate. Thus, we conclude that the velocity of MreB rotation also affects cell width, that is, the faster the MreB rotates, the thinner the cell width is.