Diversity of bacteria associated with the coral Pocillopora damicornis from the Great Barrier Reef
Diversity of bacteria associated with the coral Pocillopora damicornis from the Great Barrier Reef
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DOI：
10.1111/j.1462-2920.2005.00793.x
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发表时间：
2005-08-01
影响因子：
5.1
通讯作者：
Munn, CB
中科院分区：
文献类型：
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作者：
Bourne, DG;Munn, CB
利用非培养的分子微生物技术研究了大堡礁上的珊瑚礁建造珊瑚Pocillopora damicornis的微生物群落。三个独立的珊瑚菌落的微生物群落进行了评估，使用克隆库建设限制性片段长度多态性和系统发育分析。多样性也进行了调查，在每个单一的珊瑚群落内，使用16 S rDNA和rpoB-DGGE分析6个重复的样品在空间上。克隆文库表明，从珊瑚组织浆库检索到的大部分序列隶属于γ-变形菌。这与海水和珊瑚粘液的克隆文库形成对比，后者由α-变形菌门主导。一些检索到的克隆序列之间的珊瑚菌落保守，与以前的研究表明一个特定的微生物珊瑚协会的结果一致。重复组织浆样品的rpoB-DGGE模式低估了微生物多样性，但表明指纹是相同的同一珊瑚。这些指纹在珊瑚群落中也被保存下来。16 S rDNA-DGGE模式的复制组织浆料样品更复杂，虽然非度量多维标度（nMDS）分析显示这些带型的分组表明，一些细菌的多样性是均匀的珊瑚菌落内。从DGGE分析检索的序列数据支持克隆文库数据，因为大多数隶属关系在γ-变形菌门内。检索到的许多序列也与以前对加勒比健康珊瑚微生物多样性的研究中获得的序列密切相关。检索到与志贺氏弧菌和溶珊瑚弧菌具有高16 S rDNA序列同一性的克隆，表明这些可能是机会致病菌。两种不同的采样方法之间的检索微生物多样性的比较，注射器提取的珊瑚粘液样品和喷枪珊瑚组织浆料样品进行了研究。非度量多维缩放的克隆库数据强调，从珊瑚粘液库检索的克隆多样性更密切地反映了周围海水的多样性比相应的珊瑚组织克隆库。
The microbial community associated with the reef building coral Pocillopora damicornis located on the Great Barrier Reef was investigated using culture-independent molecular microbial techniques. The microbial communities of three separate coral colonies were assessed using clone library construction alongside restriction fragment length polymorphism and phylogenetic analysis. Diversity was also investigated spatially across six replicate samples within each single coral colony using 16S rDNA and rpoB-DGGE analysis. Clone libraries demonstrated that the majority of retrieved sequences from coral tissue slurry libraries affiliated with gamma-Proteobacteria. This contrasted with clone libraries of seawater and coral mucus, which were dominated by alpha-Proteobacteria. A number of retrieved clone sequences were conserved between coral colonies; a result consistent with previous studies suggesting a specific microbe-coral association. rpoB-DGGE patterns of replicate tissue slurry samples underestimated microbial diversity, but demonstrated that fingerprints were identical within the same coral. These fingerprints were also conserved across coral colonies. The 16S rDNA-DGGE patterns of replicate tissue slurry samples were more complex, although non-metric multidimensional scaling (nMDS) analysis showed groupings of these banding patterns indicating that some bacterial diversity was uniform within a coral colony. Sequence data retrieved from DGGE analysis support clone library data in that the majority of affiliations were within the gamma-Proteobacteria. Many sequences retrieved also affiliated closely with sequences derived from previous studies of microbial diversity of healthy corals in the Caribbean. Clones showing high 16S rDNA sequence identity to both Vibrio shiloi and Vibrio coralliilyticus were retrieved, suggesting that these may be opportunist pathogens. Comparisons of retrieved microbial diversity between two different sampling methods, a syringe extracted coral mucus sample and an airbrushed coral tissue slurry sample were also investigated. Non-metric multidimensional scaling of clone library data highlighted that clone diversity retrieved from a coral mucus library more closely reflected the diversity of surrounding seawater than a corresponding coral tissue clone library.