IMPAIRED ACETYLCHOLINE-RELEASE IN THE INFLAMED RAT INTESTINE IS T-CELL INDEPENDENT
IMPAIRED ACETYLCHOLINE-RELEASE IN THE INFLAMED RAT INTESTINE IS T-CELL INDEPENDENT
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DOI：
10.1152/ajpgi.1992.263.2.g198
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发表时间：
1992-08-01
影响因子：
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通讯作者：
ERNST, PB
中科院分区：
文献类型：
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作者：
COLLINS, SM;BLENNERHASSETT, P;ERNST, PB
我们研究了感染旋毛虫6天前大鼠肌丛纵肌制剂抑制[H-3]乙酰胆碱（[H-3]ACh）释放的机制。kcl诱导的[H-3]乙酰胆碱释放在空肠中被抑制73%，而在无虫的回肠中被抑制76%，这表明寄生虫在管腔中的局部存在并不是抑制肌肠丛乙酰胆碱释放的先决条件。倍他米松（3 mg.kg-1）治疗大鼠。在感染的第1天（第1天），抑制了感染大鼠空肠中[H-3]乙酰胆碱释放的急性炎症反应，降低了[H-3]乙酰胆碱释放的抑制（从73%降至22%）。这一发现与炎症过程抑制乙酰胆碱释放的假设是一致的。在T螺旋体感染的裸胸大鼠中也发现了[H-3]乙酰胆碱释放的明显抑制，这表明缺乏功能性T淋巴细胞。因此，乙酰胆碱释放的变化不依赖于T淋巴细胞。结合我们之前发表的研究表明，该模型中肌肉功能的改变依赖于T细胞，本研究的结果表明，炎症反应的不同成分介导了螺旋体感染大鼠平滑肌和肌肠神经的变化。
We investigated mechanisms underlying the suppression of [H-3]acetylcholine ([H-3]ACh) release from myenteric plexus-longitudinal muscle preparations of rats infected 6 days previously with Trichinella spiralis. There was a 73% suppression of KCl-evoked release of [H-3]ACh in the jejunum, and a 76% suppression was observed in the worm-free ileum, indicating that the local presence of the parasite in the lumen is not prerequisite for the suppression of ACh release from the myenteric plexus. Treatment of rats with betamethasone (3 mg.kg-1.day-1 ip) during the infection prevented the acute inflammatory response and attenuated the suppression of [H-3]Ach release (from 73 to 22%) in the jejunum of infected rats. This finding is consistent with the hypothesis that the suppression of ACh release occurs as a result of the inflammatory process. Marked suppression of [H-3]ACh release was also seen in T spiralis-infected nude athymic rats, which were shown to lack functioning T lymphocytes. Thus changes in ACh release are not dependent on T lymphocytes. Taken in conjunction with our previously published study showing that altered muscle function in this model is T cell dependent, the results of the present study indicate that different components of the inflammatory response mediate changes in smooth muscle and myenteric nerves in the T. spiralis infected rat.