Systematic screening and characterization of flavonoid glycosides in Carthamus tinctorius L. by liquid chromatography/UV diode-array detection/electrospray ionization tandem mass spectrometry
Systematic screening and characterization of flavonoid glycosides in Carthamus tinctorius L. by liquid chromatography/UV diode-array detection/electrospray ionization tandem mass spectrometry
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DOI：
10.1016/j.jpba.2007.10.036
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发表时间：
2008-02-13
影响因子：
3.4
通讯作者：
Liang, Xin-miao
中科院分区：
文献类型：
--
作者：
Jin, Yu;Xiao, Yuan-sheng;Liang, Xin-miao
中药是一个复杂的系统，由众多化合物组成，其含量和理化性质差异显着。本文建立了基于目标分子量的筛选方法，对红花中的黄酮苷进行表征。分别设计了苷元和聚糖的筛选表，以便自由选择和组合。筛选采用灵敏度和选择性较高的多反应监测（MRM）扫描方式，有利于微量成分的表征。最终筛选出77个黄酮苷，并采用正负离子模式串联质谱法对其结构进行了表征。通过MS中的断裂规则阐明了糖基化模式、糖苷配基、聚糖部分的序列和/或糖苷间连接以及糖基化位置。该方法筛选出的众多化合物显示了植物次生代谢物的结构多样性，有目的的系统筛选和随后的结构表征为中药的化学组成提供了更多信息。 (c) 2007 Elsevier B.V. 保留所有权利。
The traditional Chinese medicine (TCM) is a complex system, which always consists of numerous compounds with significant difference in the content and physical and chemical properties. In this paper, a screening method based on target molecular weights was developed to characterize the flavonoid glycosides in the flower of Carthamus tinctorius L. The screening tables of aglycone and glycan were designed, respectively, in order to select and combine freely. The multiple reaction monitoring (MRM) scan mode with higher sensitivity and selectivity was adopted in the screening, which benefit the characterization for the minor components. Seventy-seven flavonoid glycosides were screened out finally, and their structures were characterized by tandem mass spectrometric method in both positive and negative ion modes. The glycosylation mode, aglycone, sequence and/or the interglycosidic linkages of the glycan portion and glycosylation position were elucidated by the fragmentation rule in the MS. Numerous compounds screened out with this method showed the structure variety in secondary plant metabolites, and the purposeful screening systemically and subsequent structure characterization offered more information about the chemical constitutions of TCM. (c) 2007 Elsevier B.V. All rights reserved.