Analysis of anthocyanins in powdered berry extracts by planar chromatography linked with bioassay and mass spectrometry
Analysis of anthocyanins in powdered berry extracts by planar chromatography linked with bioassay and mass spectrometry
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DOI：
10.1016/j.foodchem.2013.09.038
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发表时间：
2014-03-01
期刊：
影响因子：
8.8
通讯作者：
Morlock, Gertrud E.
中科院分区：
文献类型：
--
作者：
Cretu, Georgiana C.;Morlock, Gertrud E.
采用酸化甲醇提取越橘、蓝莓、红莓、巴西莓和蔓越莓粉末提取物中的主要花色苷，并首次采用HPTLC-Vis-MS对其进行了表征。采用组合两步正相分离，首先分离花色苷，其次分离花色苷。在白色光照明（透射模式）下进行记录。在浆果粉末提取物中，特别是飞燕草素、矢车菊素、锦葵色素和芍药素的3-糖苷，进一步发现矢车菊素糖苷和相应的花青素。样品分析的重复性（n = 3）为
Major anthocyanins were extracted with acidified methanol and characterised in powdered berry extracts of bilberry, blueberry, chokeberry, acai berry and cranberry by HPTLC-Vis-MS for the first time. A combined 2-step normal phase separation was applied, first for separation of anthocyanins and secondly of anthocyanidins. Documentation was performed under white light illumination (transmission mode). In the powdered berry extracts, especially the 3-glucosides of delphinidin, cyanidin, malvidin and peonidin, further cyanidin glycosides and respective anthocyanidins were found. Calibration data revealed a good correlation, with r between 0.9988 and 0.9999. The repeatability of the sample analysis (n = 3) was