Proteomic characteristics of human sperm cryopreservation
Proteomic characteristics of human sperm cryopreservation
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DOI：
10.1002/pmic.201300225
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发表时间：
2014-02-01
期刊：
影响因子：
3.4
通讯作者：
Yin, Changjun
中科院分区：
文献类型：
--
作者：
Wang, Shangqian;Wang, Wei;Yin, Changjun
辅助生殖技术中的人类精子冷冻保存是唯一经过验证的方法，可以使不育男性成为自己的孩子的父亲。然而，冷冻和解冻会降低精子的活力、存活率和受精能力。冷冻损伤导致的精子功能障碍与蛋白质变化之间的关系尚未建立。我们通过蛋白质组学分析研究了来自9个正常精子捐献者的冷冻解冻和新鲜精子样本之间的差异蛋白质特征。两组之间27种蛋白质的丰度不同，通过Western印迹和免疫荧光染色证实了4种蛋白质的结果。这些蛋白质可能与精子的活力、存活率、顶体完整性、ATP和异柠檬酸含量、线粒体膜电位、获能、顶体反应和细胞内钙浓度有关。这些显著差异提示，冷冻后精子功能障碍可能是由于蛋白质降解和蛋白质磷酸化所致。
Human sperm cryopreservation in assisted reproductive technology is the only proven method that enables infertile men to father their own children. However, freezing and thawing reduces spermatozoon motility, viability, and fertilizing ability. An association between dysfunctional spermatozoa due to cryoinjury and protein changes has not been established. We investigated through proteomic analysis the differential protein characteristics between freeze-thawed and fresh sperm samples obtained from nine normozoospermic donors. Twenty-seven proteins differed in abundance between the two groups, and results were verified for four proteins via Western blot and immunofluorescent staining. These proteins are putatively involved in sperm motility, viability, acrosomal integrity, ATP and isocitrate content, mitochondrial membrane potential, capacitation, acrosome reaction, and intracellular calcium concentration. These marked differences suggest that dysfunctional spermatozoon after cryopreservation may be due to protein degradation and protein phosphorylation.