Kinetics of cellular uptake and retention of the benzoporphyrin derivative (BPD): relevance to photodynamic therapy
Kinetics of cellular uptake and retention of the benzoporphyrin derivative (BPD): relevance to photodynamic therapy
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苯并卟啉衍生物 (BPD) 的细胞摄取和保留动力学：与光动力疗法的相关性
DOI：
10.1117/12.199144
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发表时间：
1995
期刊：
影响因子：
--
通讯作者：
J. Levy
中科院分区：
文献类型：
--
作者：
A. Richter;H. Meadows;A. Jain;A. J. Canaan;J. Levy
使用细胞系（K562、L1210）和正常、非激活和伴刀豆球蛋白 A 激活的小鼠脾细胞研究光敏剂、苯并卟啉衍生物、单酸环 A（BPD；1-20 (mu) g/mL）的吸收和释放/保留。通过荧光（440 nm 激发，694 nm 发射）测定细胞裂解物中的 BPD 浓度。结果表明，BPD 在同一时间范围内被所有类型的细胞快速吸收和释放。 BPD 的最大值取决于细胞类型，在肿瘤细胞中最大，在正常非激活细胞中最低，在激活细胞中中等。此外，最大摄取量取决于培养基中的 BPD 浓度、孵育时间和血清的存在。所有细胞，无论类型如何，都保留了所吸收的 BPD 量的恒定比例 (20 - 30%)。该比例与孵育时间、培养基中 BPD 浓度以及血清的存在无关。然而，由于相同条件下吸收的BPD最大量存在差异，肿瘤细胞比正常细胞保留更多的BPD，活化细胞比非活化细胞保留更多BPD。保留的 BPD 能够使细胞光敏化。结果发现与体内研究相关。
Uptake and release/retention of the photosensitizer, benzoporphyrin derivative, monoacid ring A (BPD; 1 - 20 (mu) g/mL) was studied using cell lines (K562, L1210) and normal, non- activated and Concanavalin A-activated murine splenocytes. Concentrations of BPD in cell lysates were determined by fluorescence (440 nm excitation, 694 nm emission). The results showed that BPD was taken up and released rapidly by all types of cells within the same time frame. Maximum of BPD depended on the type of cells and was greatest in tumor cells, lowest in normal, non-activated cells and intermediate in activated cells. In addition, the maximum uptake depended on BPD concentration in the medium, length of incubation and presence of serum. All cells, regardless of type, retained a constant proportion (20 - 30%) of the amount of BPD taken up. This proportion was independent of length of incubation, BPD concentration in the medium and presence of serum. However, due to differences in maximum amounts of BPD taken up under the same conditions, tumor cells retained more BPD than normal cells and activated cells more than non-activated. The retained BPD was able to photosensitize the cells. The results were found to be relevant to the in vivo studies.