Gene localization by chromosome fractionation: globin genes are on at least two chromosomes and three estrogen-inducible genes are on three chromosomes.
Gene localization by chromosome fractionation: globin genes are on at least two chromosomes and three estrogen-inducible genes are on three chromosomes.
复制标题
通过染色体分级进行基因定位：珠蛋白基因位于至少两条染色体上，三个雌激素诱导基因位于三条染色体上。
DOI：
10.1073/pnas.76.3.1348
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发表时间：
1979
影响因子：
11.1
通讯作者：
H. Varmus
中科院分区：
文献类型：
--
作者：
S. Hughes;E. Stubblefield;F. Payvar;J. D. Engel;J. Dodgson;D. Spector;B. Cordell;R. Schimke;H. Varmus
Chicken metaphase chromosomes were partially purified by rate zonal centrifugation, and DNA was prepared from each of the fractions of the sucrose gradient. The DNA was digested with various restriction enzymes and subjected to electrophoresis in agarose gels. The DNA was transferred to nitrocellulose filters (as described by Southern), and the filters were hybridized with cDNA probes. Four globin genes alpha A, alpha D, beta, and rho or epsilon are located on at least two chromosomes, and three of the estrogen-inducible genes of the hen oviduct--ovalbumin, ovomucoid, and transferrin--are on three different chromosomes. These experiments also confirm our earlier assignment of the endogenous viral sequence related to Rous-associated virus-0 to a separate (and larger) chromosome than the cellular sequence related to the transforming gene of avian sarcoma virus (cellular sarc), although it now appears that cellular sarc is on a small macrochromosome, rather than on a microchromosome.
