Gene silencing of serine proteases affects melanization of Sephadex beads in Anopheles gambiae
Gene silencing of serine proteases affects melanization of Sephadex beads in Anopheles gambiae
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DOI：
10.1016/j.ibmb.2006.06.001
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发表时间：
2006-09-01
影响因子：
3.8
通讯作者：
Shi, Lei
中科院分区：
文献类型：
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作者：
Paskewitz, Susan M.;Andreev, Olga;Shi, Lei
丝氨酸蛋白酶在激活酚氧化酶原（proPO）（黑色素产生的关键酶）中发挥重要作用。我们测试了五个片段结构域丝氨酸蛋白酶基因表达敲低对冈比亚按蚊非生物靶标黑化的影响。CLIPB4的敲低导致Sephadex珠显著缺乏黑化，而CLIPB8的敲低导致向不完全黑化珠的强烈转变。CLIPB1、B9和B10的敲除具有较小的影响。CLIPB4和CLIPB8是用于激活proPO酶级联中的酶的强候选物。(c)2006爱思唯尔有限公司保留所有权利。
Serine proteases play an important role in activation of prophenoloxidase (proPO), a critical enzyme in the production of melanin. We tested the effect of knockdown of gene expression for five clip domain serine proteases on melanization of abiotic targets in Anopheles gambiae. Knockdown of CLIPB4 resulted in a striking lack of melanization of Sephadex beads while knockdown of CLIPB8 caused a strong shift towards incompletely melanized beads. Knockdown of CLIPB1, B9 and B10 had lesser effects. CLIPB4 and CLIPB8 are strong candidates for activating enzymes in the proPO enzymatic cascade. (c) 2006 Elsevier Ltd. All rights reserved.