Autologous platelet-rich plasma isolated using the Haemonetics Cell Saver 5 and Haemonetics MCS+ for the preparation of platelet gel
Autologous platelet-rich plasma isolated using the Haemonetics Cell Saver 5 and Haemonetics MCS+ for the preparation of platelet gel
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DOI：
10.1046/j.1423-0410.2001.00099.x
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发表时间：
2001-10-01
期刊：
影响因子：
2.7
通讯作者：
Valeri, CR
中科院分区：
文献类型：
--
作者：
O'Neill, EM;Zalewski, WM;Valeri, CR
背景与目的比较了三种用Haemonetics Cell Saver 5分离富血小板血浆（PRP）的方法和一种用Haemonetics MCS+分离富血小板血浆的方法。PRP含有血小板和纤维蛋白原，它们用于止血剂的制备。材料与方法使用Haemonetics Cell Saver 5时，从30名正常志愿献血者中各取500 ml血液加入70 ml柠檬酸盐-磷酸盐-葡萄糖（CPD）抗凝剂中。在另外14名正常志愿者中，使用Haemonetics MCS+，用柠檬酸葡萄糖（ACD）与血液比例为1:9的血小板提取法分离PRP。在另一项研究中，另外30名志愿者的cpd抗凝全血被用来测量血浆和低温沉淀中的纤维蛋白原水平。结果：使用造血细胞可以收集到大量的PRP。节省5比使用Haemonetics MCS+。使用Haemonetics MCS+分离的PRP的血小板浓度和血小板总数均高于使用Haemonetics Cell Saver 5分离的PRP，但四种方法的血小板浓度和PRP体积存在差异。血浆中平均纤维蛋白原水平为253 mg % +/- 47 (SD)，低温沉淀中平均纤维蛋白原水平为1085 mg % +/- 304 （SD）。结论血小板凝胶制备中PRP分离的最合适方法取决于所要进行的具体手术和患者的需要。
Background and Objectives We compared three methods of isolating platelet-rich plasma (PRP) using the Haemonetics Cell Saver 5 and one method of isolating PRP by plateletpheresis using the Haemonetics MCS+. PRP contains both platelets and fibrinogen, which are used in the preparation of haemostatic agents.Materials and Methods When the Haemonetics Cell Saver 5 was used, 500 ml of blood from each of 30 normal volunteer donors was collected into 70 ml of citrate-phosphate-dextrose (CPD) anticoagulant. In a further 14 normal volunteers, the Haemonetics MCS+ was used to isolate PRP by plateletpheresis using an acid citrate dextrose (ACD) to blood ratio of 1 : 9. In a separate study, CPD-anticoagulated whole blood from another 30 volunteers was used for measurement of fibrinogen levels in the plasma and cryoprecipitate.Results, A larger volume of PRP can be collected using the Haemonetics Cell.Saver 5 than by using the Haemonetics MCS+. The platelet concentration and the total number of platelets were higher in the PRP isolated using the Haemonetics MCS+ than in the PRP isolated by the three methods used with the Haemonetics Cell Saver 5, with differences in platelet concentration and PRP volume among the four methods. The mean fibrinogen level in the plasma was 253 mg % +/- 47 (SD) and in the, cryoprecipitate, was 1085 mg O% +/- 304 (SD).Conclusions The, most appropriate method of PRP isolation for preparation of platelet gel is dependent upon the specific surgical procedure to be undertaken and the patient's needs.