Construction of liposomes mimicking cell membrane structure through Frame-Guided Assembly strategy.
Construction of liposomes mimicking cell membrane structure through Frame-Guided Assembly strategy.
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DOI：
10.1002/anie.202005334
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发表时间：
2020-05
影响因子：
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通讯作者：
Chao Wang;Jiafang Piao;Yujie Li;Xiancheng Tian;Yuanchen Dong;Dongsheng Liu
中科院分区：
文献类型：
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作者：
Chao Wang;Jiafang Piao;Yujie Li;Xiancheng Tian;Yuanchen Dong;Dongsheng Liu
真核细胞的形状由与膜蛋白相关的细胞骨架决定，然而，具有结构稳定性的整体形态如何产生和维持的详细机制仍不完全清楚。在这里，我们基于框架引导组装（FGA）策略，通过筛选一系列跨膜肽作为主导疏水基团（LHG），成功制备了与真核细胞结构组成相似的异质脂质体。结果表明，LHG 的构象和跨膜模式在 FGA 过程中起主导作用。形成的FGA脂质体具有良好的稳定性，这可能进一步为细胞骨架-膜蛋白-脂质双层模型提供证据。利用FGA脂质体的生物相容性和稳定性，还可用于制备新型药物递送载体，在诊断成像和癌症治疗应用中具有广阔的前景。
Eukaryotic cell shapes are determined by the cytoskeleton associating to membrane proteins, however, the detailed mechanism of how the integral morphologies with structural stability is generated and maintained is still not fully understood. Here, based on the Frame-Guided Assembly (FGA) strategy, we successfully prepared hetero-liposomes with similar structural composition to eukaryotic cells by screening a series of transmembrane peptides as the leading hydrophobic groups (LHGs). It was demonstrated that the conformation and transmembrane mode of the LHGs played dominant roles during the FGA process. The FGA liposomes were formed with excellent stability, which may further provide evidence for the cytoskeleton-membrane protein-lipid bilayer model. Taking advantage of the biocompatibility and stability, the FGA liposomes were also applied to prepare novel drug delivery vehicles, which is promising in diagnostic imaging and cancer therapy applications.