Viral load of patients with hantavirus pulmonary syndrome in Argentina
Viral load of patients with hantavirus pulmonary syndrome in Argentina
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DOI：
10.1002/jmv.24260
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发表时间：
2015-11-01
影响因子：
12.7
通讯作者：
Julieta Padula, Paula
中科院分区：
文献类型：
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作者：
Maria Bellomo, Carla;Clara Pires-Marczeski, Fanny;Julieta Padula, Paula
汉坦病毒引起严重的疾病，包括肺炎，导致住院治疗，往往死亡。目前尚无特效治疗方法。汉坦病毒的发病机制还不清楚，但很可能涉及病毒介导和宿主介导的机制。本研究的目的是将汉坦病毒肺综合征病例和汉坦病毒感染个体样本中的病毒载量与临床流行病学参数和疾病结局相关联。分析了可能与病毒载量相关的变量。回顾性研究包括73例病例或家庭接触者，具有不同的临床演变。逆转录和真实的时间聚合酶链反应测定病毒载量。血液病毒RNA水平与疾病严重程度之间无统计学显著相关性。然而，病毒载量与IgG应答呈负相关，具有统计学显著性。安第斯山脉病毒南方系感染者病毒RNA水平明显增高，拉古纳内格拉病毒感染者病毒RNA水平明显降低。这些结果表明，感染病毒的基因型与疾病的严重程度，高病毒载量与低特异性IgG反应。性别、年龄、疾病严重程度与病毒载量无关。进一步的调查增加显着的案件数量，也限制了变量进行研究是必要的。J. Med. Virol. 87：1823-1830，2015. (c)2015年威利期刊公司
Hantavirus causes severe illness including pneumonia, which leads to hospitalization and often death. At present, there is no specific treatment available. The hantavirus pathogenesis is not well understood, but most likely both virus-mediated and host-mediated mechanisms, are involved. The aim of this study was to correlate viral load in samples of hantavirus pulmonary syndrome cases and hantavirus infected individuals, with clinical epidemiological parameters and disease outcome. The variables that could potentially be related with viral load were analyzed. The retrospective study included 73 cases or household contacts, with different clinical evolution. Viral load was measured by reverse-transcription and real time polymerase chain reaction. There was no statistically significant association between blood viral RNA levels and severity of disease. However, viral load was inversely correlated with IgG response in a statistically significant manner. The level of viral RNA was significantly higher in patients infected with Andes virus South lineage, and was markedly low in persons infected with Laguna Negra virus. These results suggest that the infecting viral genotype is associated with disease severity, and that high viral load is associated with a low specific IgG response. Sex, age and disease severity were not related with viral load. Further investigations increasing strikingly the number of cases and also limiting the variables to be studied are necessary. J. Med. Virol. 87:1823-1830, 2015. (c) 2015 Wiley Periodicals, Inc.