Protective effects of dieckol isolated from Ecklonia cava against high glucose-induced oxidative stress in human umbilical vein endothelial cells
Protective effects of dieckol isolated from Ecklonia cava against high glucose-induced oxidative stress in human umbilical vein endothelial cells
复制标题
DOI：
10.1016/j.tiv.2009.11.002
复制
发表时间：
2010-03-01
影响因子：
3.2
通讯作者：
Jeon, You-Jin
中科院分区：
文献类型：
--
作者：
Lee, Seung-Hong;Han, Ji-Sook;Jeon, You-Jin
使用易受氧化应激影响的人脐静脉内皮细胞 (HUVEC) 研究了 Dieckol（从 Ecklonia cava (E. cava) 中纯化的一种叶皮单宁多酚化合物）对抗高葡萄糖诱导的氧化应激的作用。高葡萄糖(30 mM)处理诱导HUVEC细胞死亡，但浓度为10或50 μg/ml的dieckol可显着抑制高葡萄糖诱导的细胞毒性。此外，用二烯酚治疗可剂量依赖性地减少硫代巴比妥酸反应物质（TBARS）、细胞内活性氧（ROS）的产生以及高葡萄糖导致的一氧化氮水平的增加。此外，高葡萄糖水平诱导HUVEC中诱导型一氧化氮合酶（iNOS）、环氧合酶-2（COX-2）和核因子κB（NF-κB）蛋白的过度表达，但dieckol处理减少了这些蛋白的过度表达。这些发现表明，迪克尔是一种潜在的治疗剂，可以减少与糖尿病相关的高血糖诱导的氧化应激造成的损害。 (C) 2009 Elsevier Ltd. 保留所有权利。
The effect of dieckol, one of phlorotannin polyphenol compound purified from Ecklonia cava (E. cava) against high glucose-induced oxidative stress was investigated using human umbilical vein endothelial cells (HUVECs), which is susceptible to oxidative stress. High glucose (30 mM) treatment induced HUVECs cell death, but dieckol, at concentration 10 or 50 mu g/ml, significantly inhibited the high glucose-induced cytotoxicity. Furthermore, treatment with dieckol dose-dependently decreased thiobarbituric acid reactive substances (TBARS), intracellular reactive oxygen species (ROS) generation and nitric oxide level increased by high glucose. In addition, high glucose levels induced the overexpressions of inducible nitric oxide synthase (iNOS), cyclooxygenase-2 (COX-2) and nuclear factor-kappa B (NF-kappa B) proteins in HUVECs, but dieckol treatment reduced the overexpressions of these proteins. These findings indicate that dieckol is a potential therapeutic agent that will reduce the damage caused by hyperglycemia-induced oxidative stress associated with diabetes. (C) 2009 Elsevier Ltd. All rights reserved.