Dual Polarization Modulation: A Real-Time, Spectral-Multiplex Separation of Circular Dichroism from Linear Birefringence Spectral Intensities
Dual Polarization Modulation: A Real-Time, Spectral-Multiplex Separation of Circular Dichroism from Linear Birefringence Spectral Intensities
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双偏振调制：圆二色性与线性双折射光谱强度的实时光谱复用分离
DOI：
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发表时间：
2000
期刊：
影响因子：
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通讯作者：
L. Nafie
中科院分区：
文献类型：
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作者：
L. Nafie
提出了一种实时、光谱复用的方法，用于圆二色谱（CD）光谱和线性双折射（LB）光谱的完全分离。该方法被称为双偏振调制（DualPolarizationModulation，DPL），涉及在CD样品之后引入第二个偏振调制源。如在常规CD光谱仪中，第一偏振调制源位于样品之前。与两个偏振调制频率中的每一个同相的检测器处的强度信号被同时并行地解调，并且相反地电子组合，以通过实时抵消来消除LB光谱。消除的准确性可以通过电子方式进行调整，而无需改变仪器的光学对准。该方法允许基线校正的CD光谱进行测量，而无需随后的CD背景测量。
A real-time, spectral-multiplex method for the complete separation of circular dichroism (CD) spectra from linear birefringence (LB) spectra is presented. The method, called dual polarization modulation (DPM), involves the introduction of a second source of polarization modulation after the CD sample. The first source of polarization modulation, as in conventional CD spectrometers, is located before the sample. Intensity signals at the detector in phase with each of the two polarization modulation frequencies are demodulated simultaneously in parallel and combined electronically in opposition to eliminate the LB spectrum by real-time cancellation. The accuracy of the cancellation can be adjusted electronically without the need to change the optical alignment of the instrument. The DPM method permits baseline-corrected CD spectra to be measured without the need for a subsequent CD background measurement.