Solid state fermentation process coupled biological pretreatment with cellulase production by Piptoporus betulinus for enhanced cellulose hydrolysis
Solid state fermentation process coupled biological pretreatment with cellulase production by Piptoporus betulinus for enhanced cellulose hydrolysis
复制标题
固态发酵工艺将生物预处理与桦木孔菌生产纤维素酶相结合，以增强纤维素水解
DOI：
10.1007/s10570-019-02359-3
复制
发表时间：
2019-04-01
期刊：
影响因子：
5.7
通讯作者：
Xue, Huiting
中科院分区：
文献类型：
--
作者：
Li, Guanhua;Zhang, Huifang;Xue, Huiting
为了实现木质纤维素乙醇的经济可行性，提出了利用桦木孔菌生产纤维素酶和预处理的一体化工艺。栽培24天后，稻草重量和半纤维素最大损失分别为21.25%和24.79%。纤维素被轻微降解并暴露。此外，改性木质素导致木质素和半纤维素之间的相互作用较弱。分别在第 12 天和第 18 天获得 CMCase 活性（58.37 U g−1 底物）和 FPase 活性（7.43 U g−1 底物）的优化酶产量。而稻草还原糖产量在第24天时获得最高（392.96 mg g−1），比纤维素酶用量减少37.15%的对照提高了2.82倍。在这项研究中，预处理、纤维素酶生产和酶水解被耦合到一个过程中，这为木质纤维素乙醇生产中环保且具有成本效益的技术开辟了一条途径。图形摘要
An integrated process of cellulase production and pretreatment employing Piptoporus betulinus was proposed to accomplish economic feasibility of lignocellulosic ethanol. After 24 days of cultivation, the maximum loss of weight and hemicellulose of rice straw was 21.25% and 24.79%, respectively. The cellulose was degraded slightly and exposed. Furthermore, the modified lignin led to the weak interaction between lignin and hemicellulose. The optimized enzyme production for CMCase activity (58.37 U g−1 substrate) and FPase activity (7.43 U g−1 substrate) were obtained on the 12th day and the 18th day, respectively. Whereas, the highest reducing sugar yield of rice straw (392.96 mg g−1) was obtained on the 24th day, which was 2.82 times higher than that of the control with the reduced cellulase dosage of 37.15%. In this study pretreatment, cellulase production and enzymatic hydrolysis were coupled into one process, which opens an avenue for environmentally friendly and cost effective technology in lignocellulosic ethanol production.Graphical abstract