Methylation of the alpha-fetoprotein gene in isogenic rat hepatoma and liver cell lines.
Methylation of the alpha-fetoprotein gene in isogenic rat hepatoma and liver cell lines.
复制标题
同基因大鼠肝癌和肝细胞系中甲胎蛋白基因的甲基化。
DOI：
10.1016/0006-291x(87)91374-x
复制
发表时间：
1987
影响因子：
3.1
通讯作者：
Chiu,JF
中科院分区：
文献类型：
--
作者：
Sakuma,K;Cook,JR;Smith,CL;Chiu,JF
我们发现，在一系列同基因大鼠肝癌细胞系中，DNA 甲基化与甲胎蛋白（AFP）基因表达呈负相关。该基因的 5' 末端在产生 AFP 的细胞中广泛去甲基化，而在不产生 AFP 的细胞系中高度甲基化。糖皮质激素显着影响肝癌细胞AFP的合成。然而，用地塞米松处理的细胞系的甲基化模式与对照细胞的甲基化模式没有不同，表明糖皮质激素对AFP基因表达的作用不会改变该基因区域的DNA甲基化。
We have found that DNA methylation is inversely correlated with alpha-fetoprotein (AFP) gene expression in a series of isogenic rat hepatoma cell lines. The 5′ end of the gene is extensively demethylated in AFP-producing cells and is highly methylated in cell lines which do not produce AFP. Glucocorticoid affects markedly the synthesis of AFP in the hepatoma cells. However, methylation patterns of cell lines which were treated with dexamethasone were not different from those of control cells, indicating that glucocorticoid action on AFP gene expression does not alter DNA methylation in this region of the gene.