The TRAMP mouse as a model for prostate cancer.
The TRAMP mouse as a model for prostate cancer.
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DOI：
10.1002/0471142735.im2005s45
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发表时间：
2001-11-01
影响因子：
--
通讯作者：
Kwon, Eugene D
中科院分区：
文献类型：
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作者：
Hurwitz, Arthur A;Foster, Barbara A;Kwon, Eugene D
转基因小鼠前列腺腺癌（TRAMP）模型密切反映了人类前列腺癌的发病机制。雄性TRAMP小鼠在青春期开始后一致且自发地发展自体（原位）前列腺肿瘤。前列腺癌的发生是由于SV 40 T抗原的表达。TRAMP模型的多功能性已经通过建立几种TRAMP衍生的前列腺肿瘤细胞系来扩展，所述前列腺肿瘤细胞系可以注射到同基因雄性非转基因C57 BL/6宿主中以诱导异位前列腺肿瘤发生。使用TRAMP-C细胞系的皮下肿瘤诱导为另外两种小鼠模型提供了基础，其中第一种可用于快速筛选治疗原发性前列腺肿瘤的实验疗法，第二种用于测试靶向前列腺癌转移的连续疗法的有效性。还提供了TRAMP前列腺和肿瘤的收获和显微切割以及肿瘤的评价和评分的详细描述。
The transgenic adenocarcinoma of the mouse prostate (TRAMP) model closely mirrors the pathogenesis of human prostate cancer. Male TRAMP mice uniformly and spontaneously develop autochthonous (orthotopic) prostate tumors following the onset of puberty. Prostate cancer occurs consequent to the expression of SV40 T antigen. The versatility of the TRAMP model has been extended by establishment of several TRAMP-derived prostate tumor cells lines that can be injected into syngeneic male nontransgenic C57BL/6 hosts to induce ectopic prostate tumorigenesis. Subcutaneous tumor induction using the TRAMP-C cell lines has provided the basis for two additional murine models, the first of which can be used for rapid screening of experimental therapies for the treatment of primary prostate tumors and the second for testing the effectiveness of adjunctive therapies targeting prostate cancer metastases. Detailed descriptions for the harvesting and microdissection of TRAMP prostates and tumors, and the evaluation and scoring of tumors are also provided.