Ginsenoside Rb3 exerts antidepressant-like effects in several animal models
Ginsenoside Rb3 exerts antidepressant-like effects in several animal models
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DOI：
10.1177/0269881111415735
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发表时间：
2012-05-01
影响因子：
4.1
通讯作者：
Xiang, Hui
中科院分区：
文献类型：
--
作者：
Cui, Jihong;Jiang, Lingxi;Xiang, Hui
人参总皂苷已被证明具有抗抑郁的治疗作用。我们报告了总人参皂苷的主要活性成分，人参皂苷Rb3 (Rb3)，它可能具有抗抑郁的作用。通过强迫游泳实验、悬尾实验和习得性无助实验，我们发现Rb3在强迫游泳和悬尾实验中对小鼠具有显著的抗静止作用，并减少了习得性无助实验中逃跑失败的次数。在利血平诱导的综合征模型中，Rb3减轻了体温过低、眼睑下垂和运动障碍。在慢性轻度应激模型中，慢性Rb3给药逆转了运动活动、新奇抑制摄食和蔗糖偏好的下降。此外，我们进行了神经化学测试，以支持我们的假设，即生化变化（即脑源性神经营养因子和单胺神经递质5-羟色胺、多巴胺和去甲肾上腺素）参与了Rb3的抗抑郁样作用。最后，我们利用全细胞膜片钳记录发现，Rb3灌注激活了体感觉皮层内神经元的动作电位传递，三七叶提取物总皂苷阻断了动作电位传递。本研究为Rb3类抗抑郁作用的作用机制提供了证据。
Total ginsenosides have been shown to have therapeutic actions as antidepressants. We report a major active ingredient of total ginsenosides, the ginsenoside Rb3 (Rb3), which may have antidepressant-like effects. Using the forced swim test, tail suspension test, and learned helplessness procedure, we found that Rb3 had significant anti-immobility effects in mice in the forced swim and tail suspension tests and reduced the number of escape failures in the learned helplessness procedure. In a reserpine-induced syndrome model, Rb3 attenuated hypothermia, palpebral ptosis, and akinesia. In the chronic mild stress model, chronic Rb3 administration reversed the decrease in locomotor activity, novelty-suppressed feeding, and sucrose preference. Furthermore, neurochemical tests were performed to support our hypothesis that biochemical variations (i.e. brain-derived neurotrophic factor and the monoamine neurotransmitters 5-hydroxytryptamine, dopamine, and norepinephrine) are involved in Rb3's antidepressant-like effects. Finally, we found, using whole-cell patch-clamp recordings, that the action potential transmission in neurons within the somatosensory cortex was excited by Rb3 perfusion and blocked with Panax notoginseng total saponins extracted from leaves. This study provides evidence for the mechanism of action of the antidepressant-like effects of Rb3.