Phenotypic and genetic characterization of antimicrobial resistance in Salmonella serovars isolated from retail meats in Alberta, Canada
Phenotypic and genetic characterization of antimicrobial resistance in Salmonella serovars isolated from retail meats in Alberta, Canada
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DOI：
10.1016/j.fm.2012.04.017
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发表时间：
2012-10-01
影响因子：
5.3
通讯作者：
Boerlin, Patrick
中科院分区：
文献类型：
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作者：
Aslam, Mueen;Checkley, Sylvia;Boerlin, Patrick
本研究确定了从加拿大阿尔伯塔省购买的零售肉类中分离出的沙门氏菌血清型、抗菌素耐药性（AMR）和耐药基因的流行程度。在一年期间（2007年5月至2008年4月），每周从艾伯塔省的19个人口普查部门收集样本。共采购鸡肉206份、火鸡肉91份、牛肉134份、猪肉133份等564份样品。沙门氏菌从鸡肉（40%）、火鸡（27%）和猪肉（2%）样品中被检出，而在碎牛肉中未发现沙门氏菌。分别从鸡肉、火鸡肉和猪肉中分离出21种、8种和3种不同的血清型。哈达沙门氏菌在鸡肉中最常见，而海德堡沙门氏菌在火鸡肉中最常见。总体而言，29%（32/110）的分离株对抗菌药物敏感，未发现对环丙沙星、阿米卡星和萘啶酸耐药。在56%的分离株中发现多重耐药（>= 2种抗菌素）。21%的鸡和25%的火鸡分离株对阿莫西林-克拉维酸（AMC）、头孢替弗（TIO）和头孢曲松（CRO）耐药。对四环素（TET）、链霉素（STR）或氨苄西林（AMP）的耐药与S. Hadar无条件相关，而对TET、AMP、AMC、TIO、CRO或头孢西林的耐药与S. Heidelberg无条件相关。最常见的耐药基因为strA/B（42%）、tet(A)（28%）、bla(CMY-2)（21%）和bla(TEM)（17%）。bla（CMY-2）和bla（TEM）基因与S. Heidelberg无条件相关；与S. Hadar和S. Kentucky的tet(A)和strA/B基因。strA/B基因与S. Heidelberg不相关。我们的数据表明，沙门氏菌血清型的流行率因肉类类型而异，AMR和耐药基因因沙门氏菌血清型而异。爱思唯尔有限公司版权所有版权所有。
This study determined the prevalence of Salmonella serovars, antimicrobial resistance (AMR) and resistance genes in Salmonella isolated from retail meats purchased in Alberta, Canada. Samples were collected during one year period (May 2007 April 2008) on weekly basis from 19 census divisions in Alberta. A total of 564 samples including chicken (n = 206), turkey (n = 91), beef (n = 134) and pork (n = 133) were purchased. Salmonella were recovered from chicken (40%), turkey (27%) and pork (2%) samples and was not found in ground beef. A total of 21, 8, and 3 different serovars were recovered from chicken, turkey and pork meats, respectively. Salmonella Hadar was most common in chicken whereas S. Heidelberg was common in turkey meat. Overall 29% (32/110) of isolates were susceptible to tested antimicrobials and resistance to ciprofloxacin, amikacin and nalidixic acid was not found in any isolate. Multiresistance (>= 2 antimicrobials) was found in 56% of isolates. Resistance to amoxicillin-clavulanic acid (AMC), ceftiofur (TIO), and ceftriaxone (CRO) was found in about 21% of chicken and 25% of turkey isolates. Resistance to either of tetracycline (TET), streptomycin (STR) or ampicillin (AMP) was unconditionally associated with S. Hadar but resistance to either of TET, AMP, AMC, TIO, CRO or cefoxitin was associated with S. Heidelberg. The strA/B (42% isolates), tet(A) (28% isolates), bla(CMY-2) (21% isolates) and bla(TEM) (17% isolates) were the most common resistance genes found. The bla(CMY-2) and bla(TEM) genes were unconditionally associated with S. Heidelberg; tet(A) and strA/B with S. Hadar and tet(B) gene with S. Kentucky. The strA/B genes were not associated with S. Heidelberg. Our data suggests that the prevalence of Salmonella serovars varied by the meat type and that AMR and resistance genes varied by the Salmonella serovars. Crown Copyright (C) 2012 Published by Elsevier Ltd. All rights reserved.