Identification of microbial DNA in human cancer
Identification of microbial DNA in human cancer
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DOI：
10.1186/1755-8794-2-22
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发表时间：
2009-05-08
影响因子：
2.7
通讯作者：
Yan, Hai
中科院分区：
文献类型：
--
作者：
Duncan, Christopher G.;Leary, Rebecca J.;Yan, Hai
背景：微生物与多种人类疾病有关，但仍有相当数量的临床重要微生物病原体有待发现。方法：我们建立了一种全基因组的方法，称为数字核型微生物鉴定(DK-MERBE)，用于鉴定人类疾病组织中的细菌和病毒的基因组DNA。该方法包括通过数字核型分析(DK)生成实验DNA标签库，然后使用编译的微生物DNA虚拟标签库分析存在微生物DNA的标签序列。结果：为了验证这项技术并鉴定可能与人类癌症发病机制相关的病原体，我们使用DK-Microbe检测了58例人类肿瘤标本中微生物DNA的存在，包括脑癌、卵巢癌和结直肠癌。结论：DK-Microbe可以识别人类肿瘤中未知的感染源，可进一步用于人类肿瘤和其他疾病的病原体DNA鉴定。
Background: Microorganisms have been associated with many types of human diseases; however, a significant number of clinically important microbial pathogens remain to be discovered.Methods: We have developed a genome-wide approach, called Digital Karyotyping Microbe Identification (DK-MICROBE), to identify genomic DNA of bacteria and viruses in human disease tissues. This method involves the generation of an experimental DNA tag library through Digital Karyotyping (DK) followed by analysis of the tag sequences for the presence of microbial DNA content using a compiled microbial DNA virtual tag library.Results: To validate this technology and to identify pathogens that may be associated with human cancer pathogenesis, we used DK-MICROBE to determine the presence of microbial DNA in 58 human tumor samples, including brain, ovarian, and colorectal cancers. We detected DNA from Human herpesvirus 6 (HHV-6) in a DK library of a colorectal cancer liver metastasis and in normal tissue from the same patient.Conclusion: DK-MICROBE can identify previously unknown infectious agents in human tumors, and is now available for further applications for the identification of pathogen DNA in human cancer and other diseases.