Tolerance of Bifidobacterium human isolates to bile, acid and oxygen
Tolerance of Bifidobacterium human isolates to bile, acid and oxygen
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DOI：
10.1016/j.anaerobe.2013.04.005
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发表时间：
2013-06-01
期刊：
影响因子：
2.3
通讯作者：
Aires, Julio
中科院分区：
文献类型：
--
作者：
Andriantsoanirina, Valerie;Allano, Solene;Aires, Julio
双歧杆菌是人类胃肠道微生物区系的一部分，因其具有促进健康的特性而被用作功能食品中的益生菌。然而，关于人类双歧杆菌菌株群体表现出的表型特征的数据很少。在这项研究中，我们比较了最多的独立人类肠道菌株的体外对酸、胆汁和氧气的耐受性。胆汁和酸的耐受性因物种和物种内的独立菌株而异：青春假单胞菌菌株对胆汁的耐受性最强，其次是长双歧杆菌和短双歧杆菌；长双歧杆菌、短双歧杆菌和牙假单胞菌在酸性pH下的生存水平最高。耐氧性主要分布在肠道双歧杆菌中：长杆菌、短杆菌和双歧杆菌耐氧性最高。B.青少年对酸和氧应激的敏感度最高。这项研究使我们有机会更新关于人类肠道双歧杆菌表型特征的知识。长杆菌和短杆菌对氧气、胆汁和酸胁迫的耐受性最好。基于这些生物学特性，长杆菌和短杆菌在人类益生菌的潜在选择方面表现出最大的兴趣。(C)2013爱思唯尔有限公司。保留所有权利。
Bifidobacteria are part of the human gastrointestinal microbiota and are used as probiotics in functional food products because of their health promoting properties. However, only few data are available on the phenotypic characteristics displayed by human bifidobacteria strain populations. In this study we compared the in vitro tolerance to acid, bile and oxygen of the largest number of independent human intestinal strains. Bile and acid tolerance varied among species and independent strains within a species: B. adolescentis strains were the most tolerant to bile followed by Bifidobacterium longum and B. breve; B. longum, B. breve and B. dentium showed the highest viability levels after exposure to acid pH. Oxygen tolerance was largely distributed among intestinal bifidobacteria: B. longum, B. breve and B. bifidum showed the highest oxygen tolerance. B. adolescentis showed the highest susceptibility to acid and oxygen stresses. The present study gave us the opportunity to update our knowledge about the phenotypic characteristics of human intestinal bifidobacteria. B. longum and B. breve harboured the best tolerance to oxygen, bile and acid stresses. Based on such biological characters, B. longum and B. breve species showed the highest interest in terms of potential selection of human probiotics. (C) 2013 Elsevier Ltd. All rights reserved.