The Unique Single-Stranded cfDNA Species in Retinoblastoma Patients Blood Plasma: Beyond New HPLC Technology
The Unique Single-Stranded cfDNA Species in Retinoblastoma Patients Blood Plasma: Beyond New HPLC Technology
复制标题
视网膜母细胞瘤患者血浆中独特的单链 cfDNA 种类：超越新的 HPLC 技术
DOI：
10.36648/2472-1646.5.3.63
复制
发表时间：
2019
期刊：
影响因子：
2.6
通讯作者：
D. Kuznetsov
中科院分区：
文献类型：
--
作者：
K. Ermakov;Alex;er A. Bukhvostov;er S Vedenkin;S. Stovbun;A. Dvornikov;A. Semenova;D. Kuznetsov
视网膜母细胞瘤患者血浆中有大量超短(50n-150n)单链DNA片段(6.84 ng·ml~(-1))，而正常人血浆中未见。提出了一种独创的高分辨率高效液相色谱技术来揭示和表征这一特性。为了解决这一问题，发展了一种新的分子尺寸排除-阴离子交换分析技术。它在诊断学和肿瘤发生研究中的适用性在这里进行了测验。
A significant population of ultrashort (50n-150n) single-stranded DNA fragments were found in exosome-free blood plasma of retinoblastoma patients (6.84 ng ml-1), but not in plasma of healthy donors. An original high resolution HPLC technique has been proposed to reveal and characterize this peculiarity. To solve this task, a novel molecular size exclusion-anion exchange analytical technique was developed. Its applicability to diagnostics and oncogenesis research is quizzed here.