Observations on the solitary cilium of rabbit oviductal epithelium: its motility and ultrastructure.
Observations on the solitary cilium of rabbit oviductal epithelium: its motility and ultrastructure.
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兔输卵管上皮孤立纤毛的观察：其运动性和超微结构。
DOI：
10.1002/aja.1001740407
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发表时间：
1985
期刊：
影响因子：
--
通讯作者：
Blandau,RJ
中科院分区：
文献类型：
--
作者：
Odor,DL;Blandau,RJ
在兔输卵管上皮细胞上观察到孤立纤毛。在通过相差显微镜观察的 3 日龄和 4 日龄动物的伞毛上皮组织培养中，大多数单纤毛表现出涡旋或漏斗型运动，而其他纤毛则具有通常的来回运动。初级纤毛通常被认为是不动的。特定鉴定的单个纤毛的透射电子显微镜揭示了与成熟输卵管纤毛细胞相比，单个纤毛的睫状轴和基体之间的差异。孤立纤毛的基体通常具有至少两个三角形的、条纹状的基部足突，其基端周围缺乏电子致密的卫星材料，并且偶尔有条纹状的细根。相比之下，成熟纤毛细胞的纤毛只有一只基足，表现出大量电子致密的卫星物质，并且缺乏细根。单纤毛的横截面显示微管模式不同于正常纤毛的常见9+2轴丝复合体，包括9+0、10+2单线、7+2双线、以及8+1双线和2单线；确实有通常的 9 + 2 排列。我们假设多个基底足突的存在可能是观察到的涡旋运动的原因。初级纤毛比正常纤毛短；最长的测量长度为 1.86 μm，底部宽度为 0.28 μm，尖端宽度为 0.14 μm。根据光学显微镜、扫描电子显微镜和透射电子显微镜观察，这种孤立纤毛在组织培养的出生后3至4天的兔子以及切除卵巢和切除卵巢/黄体酮治疗的成年动物的输卵管组织中比在发情、排卵或切除卵巢/雌二醇治疗的兔子中更常见。
Solitary cilia have been observed on rabbit oviductal epithelial cells. In tissue cultures of fimbrial epithelium of 3‐ and 4‐day‐old animals observed by phase microscopy, most of these single cilia exhibited a vortical or funnel‐type movement while others had the usual to‐and‐fro motility. Primary cilia are usually considered immotile. Transmission electron microscopy of specifically identified single cilia revealed differences between the ciliary shafts and basal bodies of the single cilia as compared to those of mature oviductal ciliated cells. The basal body of the solitary cilium often had at least two triangular, striated, basal foot processes, lacked electron‐dense satellite material around its basal end, and occasionally had striated rootlets. In contrast, the cilia of mature ciliated cells had only one basal foot, exhibited much electron‐dense satellite material, and lacked rootlets. Cross sections of the single cilia showed patterns of microtubules different from the usual 9 + 2 axonemal complexes of normal cilia and included 9 + 0, 10 + 2 singlets, 7 + 2 doublets, and 8 + 1 doublet and 2 singlets; one did have the usual 9 + 2 arrangement. We postulate that the presence of more than one basal foot process may be responsible for the vortical motility observed. The primary cilia are shorter than normal cilia; the longest one measured was 1.86 μm in length, 0.28 μm in width at its base, and 0.14 μm at its tip. Based on the lightmicroscopic, scanning‐electron‐microscopic and transmission‐electron‐microscopic observations, such solitary cilia were observed more frequently in the oviductal tissues of the 3‐ to 4‐day postnatal rabbits grown in tissue culture and in ovariectomized and ovariectomized/progesterone‐treated adult animals than in estrous, ovulatory, or ovariectomized/estradiol‐treated rabbits.