ROLE OF SULFHYDRYL GROUPS IN ACTIVATING ENZYMES - PROPERTIES OF ESCHERICHIA COLI LYSINE-TRANSFER RIBONUCLEIC ACID SYNTHETASE
ROLE OF SULFHYDRYL GROUPS IN ACTIVATING ENZYMES - PROPERTIES OF ESCHERICHIA COLI LYSINE-TRANSFER RIBONUCLEIC ACID SYNTHETASE
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DOI：
10.1021/bi00865a016
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发表时间：
1966-01-01
期刊：
影响因子：
2.9
通讯作者：
MCELROY, WD
中科院分区：
文献类型：
--
作者：
STERN, R;DELUCA, M;MCELROY, WD
研究了SH基团在大肠杆菌赖氨酸转移核糖核酸（t-RNA）合成酶中的作用。天然酶每摩尔含有两个活性SH基团，另外一个SH基团暴露在6[图像]尿素中。巯基试剂不抑制酶的焦磷酸-腺苷三磷酸交换活性。该酶被证明是大肠杆菌中唯一不受对汞苯甲酸酯（PMB）抑制的氨基酸激活酶；PMB和HgCl2抑制赖氨酸从酶到RNA的转移；HgCl2引起RNA吸收最大值的明显变化，这显然反映了结构的破坏。
The role of the SH groups in the lysine-transfer ribonucleic-acid (t-RNA) synthetase from E. coli has been investigated. The native enzyme contains two reactive SH groups per mole, and one additional SH group is exposed in 6 [image] urea. Sulfhydryl reagents do not inhibit the pyrophosphate-adenosine tri-phosphate exchange activity of the enzyme. This enzyme is shown to be the only amino-acid activating enzyme from E. coli that is not inhibited by p-mercuribenzoate (PMB); PMB and HgCl2 inhibit the transfer of lysine from the enzyme to the RNA; HgCl2 causes a marked shift in the absorption maxima of the RNA, which apparently reflects disruption of structure.