The molybdenum cofactor biosynthesis protein MobA from Rhodobacter capsulatus is required for the activity of molybdenum enzymes containing MGD, but not for xanthine dehydrogenase harboring the MPT cofactor
The molybdenum cofactor biosynthesis protein MobA from Rhodobacter capsulatus is required for the activity of molybdenum enzymes containing MGD, but not for xanthine dehydrogenase harboring the MPT cofactor
复制标题
DOI：
10.1111/j.1574-6968.1999.tb13574.x
复制
发表时间：
1999-05-15
影响因子：
2.1
通讯作者：
Klipp, W
中科院分区：
文献类型：
--
作者：
Leimkühler, S;Klipp, W
分析了荚膜红杆菌中不同钼辅助因子对钼酶的需要量。MobA是DMSO还原酶和硝酸还原酶活性所必需的，这两种酶都含有钼酸蝶呤鸟嘌呤二核苷酸辅助因子（MGD），但对含有钼酸蝶呤辅助因子的活性黄嘌呤脱氢酶则不是。与大肠杆菌和球形大肠杆菌的nob位点相反；在荚膜菌中，mobB基因并不位于mobA的下游。mobA基因在低水平下组成表达，即使在高MGD需求的条件下，也没有观察到mobA表达的增加。(C) 1999年欧洲微生物学会联合会。- Elsevier Science B.V.出版，版权所有。
The requirement of MobA for molybdoenzymes with different molybdenum cofactors was analyzed in Rhodobacter capsulatus. MobA is essential for DMSO reductase and nitrate reductase activity, both enzymes containing the molybdopterin guanine dinucleotide cofactor (MGD), but not for active xanthine dehydrogenase,, harboring the molybdopterin cofactor. In contrast to the nob locus of Escherichia coli and R. sphaeroides; the mobB gene is not located downstream of mobA in R. capsulatus. The mobA gene is expressed constitutively at low levels and no increase in mobA expression could be observed even under conditions of high MGD demand. (C) 1999 Federation of European Microbiological Societies.-Published by Elsevier Science B.V. All rights reserved.