LOCALIZATION OF MUCIN (MUC2 AND MUC3) MESSENGER-RNA AND PEPTIDE EXPRESSION IN HUMAN NORMAL INTESTINE AND COLON-CANCER
LOCALIZATION OF MUCIN (MUC2 AND MUC3) MESSENGER-RNA AND PEPTIDE EXPRESSION IN HUMAN NORMAL INTESTINE AND COLON-CANCER
复制标题
DOI：
10.1016/0016-5085(94)90057-4
复制
发表时间：
1994-07-01
期刊：
影响因子：
29.4
通讯作者：
KIM, YS
中科院分区：
文献类型：
--
作者：
CHANG, SK;DOHRMAN, AF;KIM, YS
背景/目的：几项研究报道了北方印迹数据显示粘蛋白以组织特异性方式表达。方法：采用免疫细胞化学和原位杂交技术，对人小肠、结肠和结肠癌组织中表达MUC 2和MUC 3粘蛋白的细胞类型进行鉴定。相反，识别MUC 3核粘蛋白的抗体染色杯状细胞和吸收细胞。与此相一致的是，原位杂交显示MUC 2 mRNA仅在杯状细胞中表达，而MUC 3 mRNA在杯状细胞和吸收细胞中均有表达。在几个样本中的中度分化良好的结肠癌，MUC 2andMUC 3表现出不同的模式的表达，但每个的表达水平降低与正常组织中的水平相比，有相当大的肿瘤和细胞之间的变异性使用粘蛋白抗体和互补的DNA probes.Conclusions：个别粘蛋白基因有不同的模式内的粘蛋白生产组织的表达，这表明各种粘蛋白基因产物发挥不同的功能作用。
Background/Aims:Several studies have reported Northern blot data showing that mucin is expressed in a tissue-specific manner. To determine whether expression is limited to specific cell types within these tissues requires histological analysis.Methods:Both immunocytochemistry and in situ hybridization were used to identify cell types expressing theMUC2andMUC3mucins in the human small intestine, colon, and colon carcinoma.Results:In the normal small intestine and colon, an antibody recognizing theMUC2apomucin stained goblet cells. In contrast, an antibody recognizing theMUC3apomucin stained both goblet and absorptive cells. Consistent with this, in situ hybridization showedMUC2messenger RNA (mRNA) only in goblet cells andMUC3mRNA in both goblet and absorptive cells. In several samples of moderately well-differentiated colon cancer,MUC2andMUC3showed distinct patterns of expression, but the expression level of each was reduced compared with levels in normal tissue; there was considerable tumor-to-tumor and cell-to-cell variability using both mucin antibodies and complementary DNA probes.Conclusions:Individual mucin genes have distinct patterns of expression within mucin-producing tissues, suggesting that the various mucin gene products play distinct functional roles.