Sampling of adult bees for detection of American foulbrood (Paenibacillus larvae subsp larvae) spores in honey bee (Apis mellifera) colonies
Sampling of adult bees for detection of American foulbrood (Paenibacillus larvae subsp larvae) spores in honey bee (Apis mellifera) colonies
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DOI：
10.1080/00218839.2005.11101154
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发表时间：
2005-01-01
影响因子：
1.9
通讯作者：
Fries, I
中科院分区：
文献类型：
--
作者：
Lindström, A;Fries, I
对瑞典中部一个养蜂场的59个养蜂场共489个蜂群进行了检查，这些养蜂场以前有美国恶臭（AFB）的历史。目视检查每个菌落的临床疾病状况，并估计AFB临床患病的育雏细胞数量。从其中的94个蜂群中，从育雏室和巢中收集了100只活蜜蜂的个体样本。分别从(1)所有的育雏室和(2)所有的巢室中，从每个被检查的蜂房中每个单独的蜂群中采集2个复合样本，由bb100只成蜂组成。在个体集落水平和蜂房水平采集样本，分析是否存在芽孢杆菌幼虫亚种。幼虫通过培养。在100% （n = 21）的临床患病蜂群和77%的超级蜜蜂样本中发现孢子，在82%的无临床症状蜂群的育雏室中发现孢子（n = 73）。在蜂房水平上，100%的临床患病蜂房标本呈阳性（n = 32）。在未出现临床疾病的养蜂场中，70%的超级蜂复合样本和67%的育雏室样本呈AFB阳性（n = 27）。在孵卵室和孵卵室中，蜜蜂的孢子载量仅略有不同，这表明在筛选养蜂场时，从孵卵室中取样蜜蜂是一种快速而令人满意的替代方法。
Fifty-nine apiaries with a total of 489 honey bee colonies belonging to a beekeeping operation in central Sweden with a previous history of American foulbrood (AFB) were inspected. For each colony the clinical disease status was visually inspected and the number of brood cells clinically diseased by AFB estimated. From 94 of these colonies, individual samples of > 100 live honey bees from the brood chamber and supers were collected. Two composite samples consisting of > 100 adult bees from each individual colony in each inspected apiary were also taken from (1) all brood chambers and (2) all supers, respectively. Samples were taken on both the individual colony level and the apiary level and analysed for presence of Paenibacillus larvae subsp. larvae by culturing. Spores were found in 100% (n = 21) of the bee samples from clinically diseased colonies and in 77% of the supers, and 82% of the brood chambers in colonies without clinical symptoms (n = 73). On the apiary level, 100% of the samples from clinically diseased apiaries were positive (n = 32). In the apiaries that showed no clinical disease, 70% of the composite samples from supers and 67% of the samples from brood chambers were positive for AFB (n = 27). Spore load differed only slightly between bees in brood chambers and in supers, suggesting that sampling bees from supers when screening apiaries for AFB is a fast and satisfactory alternative to sampling brood chambers.