Abundant class of human DNA polymorphisms which can be typed using the polymerase chain reaction.
Abundant class of human DNA polymorphisms which can be typed using the polymerase chain reaction.
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DOI：
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发表时间：
1989-03
影响因子：
9.8
通讯作者：
J. Weber;P. May
中科院分区：
文献类型：
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作者：
J. Weber;P. May
（dC-dA）n形式的散布的DNA元件。（dG-dT）n是人类最丰富的重复DNA家族之一。我们报告，特定的人类（dC-dA）n。（dG-dT）n块在个体之间的长度是多态的，因此代表了潜在的遗传标记的巨大的新池。文献中（dC-dA）n序列的比较。（dG-dT）n块克隆两次或更多次，在8例中的7例显示长度多态性。10（dC-dA）n的长度变化。通过使用聚合酶链反应扩增重复区段内和紧邻重复区段侧翼的DNA，然后在聚丙烯酰胺DNA测序凝胶上解析扩增的DNA，直接证明（dG-dT）n区段。与标准印迹和杂交相比，使用聚合酶链反应检测DNA多态性提供了改进的灵敏度和速度。
Interspersed DNA elements of the form (dC-dA)n.(dG-dT)n constitute one of the most abundant human repetitive DNA families. We report that specific human (dC-dA)n.(dG-dT)n blocks are polymorphic in length among individuals and therefore represent a vast new pool of potential genetic markers. Comparison of sequences from the literature for (dC-dA)n.(dG-dT)n blocks cloned two or more times revealed length polymorphisms in seven of eight cases. Variations in the lengths of 10 (dC-dA)n.(dG-dT)n blocks were directly demonstrated by amplifying the DNA within and immediately flanking the repeat blocks by using the polymerase chain reaction and then resolving the amplified DNA on polyacrylamide DNA sequencing gels. Use of the polymerase chain reaction to detect DNA polymorphisms offers improved sensitivity and speed compared with standard blotting and hybridization.