An extracellular protease with depilation activity from Streptomyces nogalator
An extracellular protease with depilation activity from Streptomyces nogalator
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发表时间：
2005-12
影响因子：
0.6
通讯作者：
P. Mitra;P. K. Chakrabartty
中科院分区：
文献类型：
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作者：
P. Mitra;P. K. Chakrabartty
从土壤中分离到一株产胞外蛋白酶的放线菌--链霉菌Ac 80。选择与野生型（1.8mg酪氨酸释放/min/mg蛋白质）相比具有更高（3.0mg酪氨酸释放/min/mg蛋白质）比活性和更低（0.9mg酪氨酸释放/min/mg蛋白质）比活性的Mt C生产蛋白酶的菌株的突变体。野生型和突变体的蛋白水解活性与它们的脱毛能力相关。将Mt C固态发酵产生的蛋白水解酶纯化至均一，并进行部分表征（SDS-PAGE测得分子量为66 kDa）。对山羊皮脱毛效果显著。
An extracellular protease-producing actinomycete, Streptomyces nogalator strain Ac 80, was isolated from soil. Mutants of the strain designated as Mt C producing protease of higher (3.0 mg tyrosine liberated/min/mg protein) and Mt L of lower (0.9 mg tyrosine liberated/min/mg protein) specific activity as compared to that of the wild type (1.8 mg tyrosine liberated/min/mg protein) were selected. Proteolytic activity of the wild type and the mutants correlated with their depilation capacities. Proteolytic enzyme produced by Mt C in solid state fermentation was purified to homogeneity, and partially characterized (molecular mass, 66 kDa by SDS- PAGE). It was found to be efficient in depilation of goatskin.