CLONING AND EXPRESSION OF STAPHYLOCOCCUS-SAPROPHYTICUS UREASE GENE-SEQUENCES IN STAPHYLOCOCCUS-CARNOSUS AND CONTRIBUTION OF THE ENZYME TO VIRULENCE
CLONING AND EXPRESSION OF STAPHYLOCOCCUS-SAPROPHYTICUS UREASE GENE-SEQUENCES IN STAPHYLOCOCCUS-CARNOSUS AND CONTRIBUTION OF THE ENZYME TO VIRULENCE
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DOI：
10.1128/iai.57.10.2998-3002.1989
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发表时间：
1989-10-01
影响因子：
3.1
通讯作者：
MARRE, R
中科院分区：
文献类型：
--
作者：
GATERMANN, S;MARRE, R
克隆了金黄色葡萄球菌（Staphylococcus saceticus）CCM 883的尿素酶基因，并在肉葡萄球菌（Staphylococcus carnosus）TM 300中表达。克隆的DNA序列的体外翻译揭示了与酶活性相关的六种多肽（70、47、29、27、20和17千道尔顿）。将所克隆的基因导入一株尿素酶阴性的突变株中。sacchariticus恢复了该菌株的毒力，证实了我们以前的建议（S. Gatermann，J. John，and R. Infect. Immun. 57：110-116，1989）该酶是该生物体的主要毒力因子，主要导致细胞病理学。
The urease gene of Staphylococcus saprophyticus CCM883 was cloned and expressed in Staphylococcus carnosus TM300. In vitro translation of the cloned DNA sequences revealed six polypeptides (of 70, 47, 29, 27, 20, and 17 kilodaltons) that were associated with enzyme activity. Introduction of the cloned genes into a urease-negative mutant of S. saprophyticus restored the virulence of this strain, confirming our previous suggestion (S. Gatermann, J. John, and R. Infect. Immun. 57:110-116, 1989) that this enzyme is a major virulence factor of the organism and contributes mainly to cystopathogenicity.