Identification of yeasts within Saccharomyces sensu stricto complex by PCR-fingerprinting
Identification of yeasts within Saccharomyces sensu stricto complex by PCR-fingerprinting
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DOI：
10.1007/s11274-007-9407-6
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发表时间：
2007-11-01
影响因子：
4.1
通讯作者：
de Morais, Marcos, Jr.
中科院分区：
文献类型：
--
作者：
Brito dos Santos, Scheila Karina;Basilio, Anna Carla Moreira;de Morais, Marcos, Jr.
生物亲缘关系使得工业上重要的严格感酵母菌复合体的物种表征变得困难。本文提出了一套基于单引物扩增反应（Single引物Amplification Reactions， SPAR）的pcr指纹标记，结合pcr -核糖分型和单基因RFLP分析，可以有效地识别单个物种，并充分表征工业分离物的杂交性质。利用这些标记，我们可以对所有6种严格感觉复合体的酵母菌进行区分，并且我们还发现了以前对某些葡萄酒酵母的分类鉴定中的错误。SPAR标记产生的独特模式可用于监测工业发酵过程中的酵母种群，并可用于检测这些环境中酵母杂交的外观。
Biological relatedness makes species characterization of the industrially important Saccharomyces sensu stricto complex difficult. In this paper we present a set of PCR-fingerprinting markers based in Single Primer Amplification Reactions (SPAR) that, together with PCR-ribotyping and single gene RFLP analysis, can effectively identify individual species and fully characterize the hybrid nature of industrial isolates. With those markers, all six yeast species of the sensu stricto complex could be discriminated and we also identified errors in the previous taxonomic characterization of certain wine yeasts. The unique patterns generated by the SPAR markers could be useful in monitoring yeast populations during industrial fermentation processes and can be used to detect the appearance of yeast hybrids in these environments.