COMPARISON OF CILIARY ACTIVITY AND VIRUS RECOVERY FROM TRACHEAS OF CHICKENS AND HUMORAL IMMUNITY AFTER INOCULATION WITH SEROTYPES OF AVIAN INFECTIOUS-BRONCHITIS VIRUS
COMPARISON OF CILIARY ACTIVITY AND VIRUS RECOVERY FROM TRACHEAS OF CHICKENS AND HUMORAL IMMUNITY AFTER INOCULATION WITH SEROTYPES OF AVIAN INFECTIOUS-BRONCHITIS VIRUS
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DOI：
10.2307/1589869
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发表时间：
1982-01-01
期刊：
影响因子：
1.4
通讯作者：
SNYDER, DB
中科院分区：
文献类型：
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作者：
MARQUARDT, WW;KADAVIL, SK;SNYDER, DB
用同源和异源血清型传染性支气管炎病毒（IBV）进行交叉保护试验，比较鸡气管纤毛活性和病毒回收率。该技术的验证包括关联从一些感染的鸟类获得的抗血清的中和指数。用JMK或Connecticut（Conn）IBV血清型对鸡进行气管内接种。感染组和未感染组在3周后通过相同的途径用同源和异源病毒进行攻击。JMK株提供了对同源攻击和Conn株的免疫力，如良好的纤毛活性和缺乏攻击病毒回收所示。Conn株仅提供同源保护，因为纤毛停滞发生，并且用JMK株攻击后病毒被回收。在每种情况下，免疫病毒的抗血清只能中和同源病毒。对照组一致易感且缺乏中和抗体。用方舟99血清型和IBV的最近分离株（397）进行的类似实验显示气管的完全交叉保护。在相互试验中，每种病毒的抗血清都能中和同源和异源病毒。因此，这两种病毒密切相关。纤毛活性和病毒分离之间的完全一致性表明纤毛活性是在交叉保护试验中判断气管免疫的可靠、客观的标准。
Cross-protection tests with homologous and heterologous serotypes of infectious bronchitis virus (IBV) were used to compare ciliary activity and virus recovery from tracheas of chickens. Validation of this technique included correlating the neutralization indices of antiserum obtained from some infected birds. Chickens were inoculated intratracheally with the JMK or Connecticut (Conn) IBV serotype. Infected and uninfected groups were challenged 3 wk later by the same route with homologous and heterologous virus. The JMK strain provided immunity against homologous challenge and the Conn strain, as indicated by good ciliary activity and lack of challenge virus recovery. The Conn strain provided only homologous protection as ciliostasis occurred and virus was recovered after challenge with the JMK strain. In each case, antiserum to immunizing virus neutralized only the homologous virus. Controls were uniformly susceptible and lacked neutralizing antibody. A similar experiment with the Ark 99 serotype and a recent isolate (397) of IBV revealed complete cross-protection of the tracheas. Antiserum to each virus neutralized the homologous and heterologous virus in each case in reciprocal tests. Thus, these 2 viruses are closely related. Complete agreement between ciliary activity and virus isolation indicates that ciliary activity is a reliable, objective criterion for judging tracheal immunity in cross-protection tests.