Resolution of electronic absorption bands and nucleotide binding processes in actinomycin D
Resolution of electronic absorption bands and nucleotide binding processes in actinomycin D
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放线菌素 D 中电子吸收带和核苷酸结合过程的解析
DOI：
10.1002/bip.1978.360170807
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发表时间：
1978
期刊：
影响因子：
2.9
通讯作者：
T. Krugh
中科院分区：
文献类型：
--
作者：
H. Auer;Barbara E. Pawlowski‐Konopnicki;Y. Chiao;T. Krugh
用吸收光谱、圆二色谱和圆二色谱研究了放线菌素D与模型二氧核苷的络合物。放线菌素D分别与5‘-dGMP、PDG-DT、PDT-DG、PDG-da和PDA-DG形成的络合物的圆二色谱相似。推测在溶液中，鸟嘌呤残基与放线菌素D的相互作用与结晶型1：2放线菌素D：DG络合物中的作用相同[Jain，S.C.&Sobell，H.M.(1972)J.Mol.比奥尔。68，1-20]。PDG-DC与上述配合物的CD光谱不同，与放线菌素D与小牛胸腺DNA和Poly(DG-DC)-Poly(DG-DC)形成的配合物的CD光谱相似。这些结果与PDG-DC与放线菌素D形成双星插层复合体的观点一致，DG-DC序列是多脱氧核苷酸中放线菌素D的重要结合部位。
Complexes of actinomycin D with model dexoxynucleoides have been studied by means of absorption spectroscopy and CD spectroscopy and CD spectroscopy. The CD spectra of the complexes of actinomycin D with 5′‐dGMP, pdG‐dT, pdT‐dG, pdG‐dA, and pdA‐dG, respectively, all resemble one another. It is presumed that in solution the interactions between the guanine residues and actinomycin D in these complexes are the same as found in the crystalling 1:2 actinomycin D:dG complex [Jain, S. C. & Sobell, H. M. (1972) J. Mol. Biol. 68, 1–20]. The CD spectrum of the Complexes with pdG‐dC differs from of the complexes just mentioned, and resembles those of the complexes formed by actinomycin D with calf‐thymus DNA and with poly(dG‐dC)‐poly(dG‐dC). These resulls are consisent with, the nontion that pdG‐dC froms a double‐staranded intercalated complex with actinomycin D, and that the dG‐dC sequence is an important binding site for actinomycin D in polydeoxynucleotides.