CHOLECYSTOKININ OCTAPEPTIDE-LIKE IMMUNOREACTIVITY - HISTOCHEMICAL LOCALIZATION IN RAT-BRAIN
CHOLECYSTOKININ OCTAPEPTIDE-LIKE IMMUNOREACTIVITY - HISTOCHEMICAL LOCALIZATION IN RAT-BRAIN
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DOI：
10.1073/pnas.76.1.521
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发表时间：
1979-01-01
影响因子：
11.1
通讯作者：
SNYDER, SH
中科院分区：
文献类型：
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作者：
INNIS, RB;CORREA, FMA;SNYDER, SH
采用间接免疫荧光法对大鼠脑内CCK-OP样免疫反应性进行定位。免疫组化研究的特异性是通过免疫前血清或预吸附CCK-OP的血清几乎消除染色，以及在2种不同的初级抗CCK-OP血清中实现相似的荧光模式来证明的。cck - op样荧光定位于神经元细胞体、纤维和静脉曲张终末。最密集的CCK-OP细胞集中在导水管周围灰质和下丘脑背内侧。大量的细胞和纤维存在于内侧/背侧和嗅周皮层；在海马锥体层和中缝背中发现的细胞群更有限。
Cholecystokinin octapeptide (CCK-OP)-like immunoreactivity was localized in the rat brain by using the indirect immunofluorescence method. Specificity in immunohistochemical studies was demonstrated by the virtual elimination of staining with pre-immune sera or sera preadsorbed with CCK-OP and by the achievement of similar fluorescent patterns with 2 different primary anti-CCK-OP sera. CCK-OP-like fluorescence was localized in neuronal cell bodies, fibers and varicose terminals. The most dense collections of CCK-OP cells occurred in the periaqueductal gray and in the dorsomedial hypothalamus. Substantial numbers of cells and fibers were present in the medial/dorsal and perirhinal cortex; more limited groups of cells were found in the pyramidal layer of the hippocampus and in the dorsal raphe.