Cerebellar synaptic protein expression in schizophrenia
Cerebellar synaptic protein expression in schizophrenia
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DOI：
10.1016/s0306-4522(01)00141-5
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发表时间：
2001-01-01
期刊：
影响因子：
3.3
通讯作者：
Harrison, PJ
中科院分区：
文献类型：
--
作者：
Eastwood, SL;Cotter, D;Harrison, PJ
皮质-皮质下-小脑神经回路被认为在精神分裂症的病理生理学中很重要。这项研究调查是否有小脑突触的变化，伴随其假定参与疾病。我们测量了三种突触蛋白（突触素，复合蛋白I和复合蛋白II）的表达在小脑皮质的16例精神分裂症患者和16名对照组使用原位杂交组织化学和免疫放射自显影。复合蛋白I和II分别主要由抑制性和兴奋性神经元表达。在精神分裂症中，突触素mRNA减少，复合蛋白II及其mRNA也减少。复合蛋白I mRNA和蛋白水平不变。大鼠小脑中的mRNA表达不受2周抗精神病药物（氟哌啶醇、氯丙嗪、利培酮、奥氮平或氯氮平）的影响。通过破坏神经回路，这些改变可能导致小脑功能障碍，被认为是发生在疾病中。(C)2001年IBRO。由爱思唯尔科技有限公司出版。保留所有权利。
A cortico-subcortico-cerebellar neural circuit has been postulated to be important in the pathophysiology of schizophrenia. This study investigated whether there are synaptic changes in the cerebellum to accompany its putative involvement in the disorder. We measured the expression of three synaptic proteins (synaptophysin, complexin I and complexin II) in the cerebellar cortex of 16 subjects with schizophrenia and 16 controls using in situ hybridisation histochemistry and immunoautoradiography. Complexin I and II are expressed predominantly by inhibitory and excitatory neurones respectively. In schizophrenia, synaptophysin mRNA was decreased, as was complexin II and its mRNA. Complexin I mRNA and protein levels were unaltered. Expression of the mRNAs in the rat cerebellum was unaffected by 2 weeks administration of antipsychotic drugs (haloperidol, chlorpromazine, risperidone, olanzapine or clozapine).We conclude that there is synaptic pathology in the cerebellum in schizophrenia. By disrupting neural circuits, the alterations may contribute to the cerebellar dysfunction thought to occur in the disorder. (C) 2001 IBRO. Published by Elsevier Science Ltd. All rights reserved.